CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



225 



successive days for 10 minutes, taking 



care not to overheat. 

 Use: Enrichment of lactose fermenters. 



The author reported that the addition of 



KI may tend to delay the anaerobic 



growth while not interfere with aerobic 



multiplication. 

 Added nutrients: The author added 0.5 g. 



glucose, 1.0 g. sucrose or 0.5 g. lactose. 

 Reference: MacConkey (1905 p. 338). 



770. Klimenko's Basal Glycerol Bouillon 



Constituents: 



1. Peptone bouillon 1000.0 cc. 



2. Glycerol (1 .0%) 10.0 g. 



Preparation : 



(1) Preparation of peptone bouillon not 

 given. 



(2) Add (1.0%) glycerol to (1). 



(3) Mix equal parts of (2) and one of the 

 added nutrients. 



Sterilization: Not specified. 



Use: Cultivation of whooping cough bacil- 

 lus. Author reported that the organism 

 grows first on the surface and then on the 

 bottom of the tube. 



Added nutrients: The author mixed equal 

 parts of one of the following and the 

 basic solution: 

 ascitic fluid 

 blood serum 

 defibrinated blood 



Reference: Klimenko (1909 p. 312). 



771. Cantani's Basal Ascitic Fluid Bouillon 



Constituents: 



1. Bouillon 



2. Ascitic fiuid 



3. Glycerol 

 Preparation : 



(1) Mix equal parts of glycerol and one of 

 the added nutrients. Storing the 

 material with the glycerol tends to 

 sterilize the material. 



(2) After some time test the sterility 

 of (1). 



(3) Exact composition of bouillon not 

 given. 



(4) Tube (3). 



(5) Add 6 parts of sterile ascitic fluid to 

 1 part (2). 



(6) Add 0.5 to 0.75 cc. of (5) to each 

 sterile tube of (4). 



Sterilization: Method not specified. 



Use: Cultivation of highly parasitic and 



pathogenic bacteria. 

 Added nutrients and variants: 



(a) The author mixed glycerol with equal 

 parts of urine, pus, milk, egg white or 

 other albuminous fluids. 



(b) The author used a mixture of equal 

 parts of ascitic fluid and glycerol 

 instead of ascitic fluid in step (5) 

 above. 



Reference: Cantani (1910 p. 472). 



772. Buerger's Basal Ascitic Fluid Bouillon 

 Constituents: 



1. Bouillon sugar free 1000.0 cc. 



2. Ascitic fluid 333.0 cc. 



3. Litmus 

 Preparation: 



(1) Preparation of sugar free bouillon 

 not given. 



(2) Add litmus solution to (1) until a 

 desirable color is obtained. 



(3) Add I volume of sterile ascitic fluid to 

 sterile (2). 



(4) Add sterile watery concentrated solu- 

 tions of one of the added nutrients to 

 (3) until a 1.0% strength is obtained. 



(5) Tube into sterile tubes. 



(6) Incubate in incubator to test sterility. 

 Sterilization: Method of sterilization of (2) 



not given. Sterilize the solution of 

 added nutrients by heating on each of 3 

 successive days. 



Use: To determine the fermentation of 

 carbohydrates, alcohols, etc., by bac- 

 teria. Buerger used streptococci. 



Added nutrients and variants: 



(a) The author added 1.0% of one of the 

 following materials: 



arabinose dulcitol 



rhamnose sucrose 



glucose lactose 



levulose maltos 



galactose dextrin 



mannitol inulin 



(b) Elser and Huntoon prepared a sim- 

 ilar medium using one part ascitic 

 fluid with two parts bouillon. The 

 procedure was as follows: 



(1) Mix two parts sterile broth (exact 

 method of preparation or composi- 

 tion not given) with one part sterile 

 ascitic fluid. Method of steriliza- 

 tion not specified. 



