CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



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(4) Weigh the saucepan and cook for 

 about 30 minutes. 



(5) Strain thru cheese cloth, and 

 press all the liquid out in a meat 

 press. 



(6) Replace the water lost by evap- 

 oration. 



(7) Dissolve 10.0 g. of Witte's pep- 

 tone in (6). 



(8) Adjust the reaction. 



(9) Filter into flasks. 



(10) Sterilize in the autoclave at 120° 



for 10 minutes. 



(11) Tube (sterile tubes?). 



(n) Committee A. P. H. A. (1909). 



(1) Infuse 500.0 g. finely chopped lean 

 meat with 1000.0 cc. distilled 

 water for 24 hours in the ice box. 



(2) Make up the loss due to evapora- 

 tion. 



(3) Strain thru cotton flannel. 



(4) Add 1.0% peptone and heat on 

 the water bath until solution is 

 complete. 



(5) Heat over boiling water or steam 

 for 30 minutes. 



(6) Restore the loss due to evapo- 

 ration. 



(7) Add normal NaOH until the 

 reaction is +1.0%. 



(8) Boil 2 minutes over a free flame. 

 Stir constantly. 



(9) Make up the loss due to evap- 

 oration. 



(10) Filter thru absorbent cotton until 

 clear. 



(11) Retitrate and record the re- 

 action. 



(12) Tube in 10.0 cc. quantities. 



(13) Sterilize (method not given), 

 (o) Lohnis (1913). 



(1) Mix one pound of lean unsalted 

 minced meat with one liter of 

 water. 



(2) Allow to stand in a cool place for 

 12 to 24 hours. 



(3) Filter thru cloth, squeezing the 

 meat free from juice until 1 liter 

 of juice is obtained. Make up to 

 a liter by the addition of water, 

 if necessary. 



(4) Add 1.0% Witte's peptone and 

 0.5% NaCl. 



(5) Heat for 60 minutes in the steam 

 sterilizer. 



(6) Filter thru filter paper. 



(7) Add concentrated soda solution 

 until red litmus paper is turned 

 slightly blue. 



(8) Heat for 30 to 45 minutes in the 

 steam sterilizer. 



(9) Filter. 



(10) Sterilization not specified, 

 (p) Sears (1916). 



(1) Grind beef fine and obtain juice 

 in 1 liter of water (soaking or 

 standing not specified). 



(2) Dissolve 10.0 g. Witte's peptone 

 and 5.0 g. NaCl in (1). 



(3) Adjust to neutral to phenol- 

 phthalein. 



(4) Distribute in 2-500.0 cc. lots. 



(5) Sterilize at 15 pounds pressure 

 (time not specified). 



(q) Roddy (1917). 



(1) Mix 500.0 g. of minced lean beef 

 or veal in 1000.0 cc. of water. 



(2) Place in an ice box for 24 hours in 

 a shallow dish. 



(3) Skim off the fat. 



(4) Filter thru linen. 



(5) Express the juice contained in 

 the meat. 



(6) Boil for 2 hours. 



(7) Filter thru linen. 



(8) Boil again for one hour. 



(9) Add water to make up the loss 

 due to evaporation. 



(10) Add 10.0 g. of peptone and 5.0 g. 

 NaCl. 



(11) Boil for 30 minutes. 



(12) Make up the loss due to evap- 

 oration. 



(13) Adjust the reaction. 



(14) Filter while hot and again when 

 cool. 



(15) Sterilize in the autoclave, 

 (r) Roddy (1917). 



Prepared as variant (q) above, 

 step (1) thru (11). 



(12) Inoculate with a culture of Bacil- 

 lus coll. 



(13) Incubate for 24 hours at 37°. 



(14) Boil for 30 minutes. 



(15) Filter while hot and again when 

 cool. 



