CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



233 



(11) Adjust the Reaction to +1.5% by 

 the addition of NaOH or HCI. 



(12) Filter thru the same paper until 

 the medium is bright and clear. 



(13) Distribute as desired. 



(14) Sterilize in the autoclave at 

 120°C. for 30 minutes. 



(w) Dopter and Sacquepee (1921). 



(1) Add 1000.0 cc. of water to 500.0 g. 

 of finely chopped fat and tendon 

 free beef. 



(2) Allow to stand in the ice box for 

 12 hours, or heat at 50 to 55° for 

 30 minutes. 



(3) Heat slowly to boiling. 



(4) Boil slowly for 10 minutes stir- 

 ring constantly. 



(5) Press the liquid thru a clean 

 cloth. 



(6) Add 20.0 g. peptone and 5.0 g. 

 NaCl 1000.0 g. of the filtrate 

 and dissolve by shaking. 



(7) Filter thru a wetted filter paper. 



(8) Make slightly alkaline to litmus 

 by the addition of NaOH or 

 NasCOa. 



(9) Flask. 



(10) Autoclave at 117° for 15 minutes. 



(11) Filter thru filter paper. 



(12) Make up to 1000.0 cc. by the 

 addition of distilled water. 



(13) Tube or flask. 



(14) Sterilize at 115°C. for 20 minutes, 

 (x) Abbott (1921). 



(1) Add 500.0 g. of chopped lean 

 beef to 1 liter of water and soak 

 for 24 hours, kept at ice box tem- 

 perature. 



(2) Strain thru a coarse towel and 

 press until a liter of fluid is 

 obtained. 



(3) Add 10.0 g. (1.0%) dry peptone 

 and 5.0 g. (0.5%) NaCl. 



(4) Make slightly alkaline or neutral 

 (indicator not specified). 



(5) Place in a porcelain lined sauce- 

 pan, and boil over a flame. Boil 

 until all the albumin is coagulated 

 and the fluid portion is of a clear 

 pale straw color. 



(6) Filter thru a folded paper. 



(7) Sterilize by steam (method not 

 given). 



(y) Foster and Randall (1921). 



(1) Prepare beef infusion broth from 

 1000.0 cc. water, 300.0 g. lean 

 beef and 10.0 g. Parke Davis 

 & Co. peptone (Procedure not 

 given). 



(2) Initial pH = 5.0 to 9.0, changing 

 every 0.4 interval using N/10 

 or N/1 NaOH and N/10 or N/1 

 HCI. 



(3) Sterilize at 15 pounds for 15 

 minutes. 



(z) Randall and Hall (1921). 



(1) Soak ground lean beef in water 

 over night and then boil 10 

 minutes. 



(2) Remove coagulated albumin by 

 straining under pressure and 

 paper filtration of the liquid. 



(3) Add peptone (2.0%) and NaCl 

 (0.5%). 



(4) Adjust to any desired reaction. 



(5) Method of sterilization not given, 

 (aa) Ayers, Rupp and Mudge (1921). 



(1) Method of preparation or compo- 

 sition of infusion broth not 

 given. 



(2) Dissolve 40.0 g. Bacto peptone 

 in (1). 



(3) Adjust to pH = 7.5. 



(4) Method of sterilization not given, 

 (bb) Harvey (1921-22). 



(1) Mince finely fat-free beef. 



Note: Veal, chicken, ox or 

 horse heart, horse flesh, rabbit 

 flesh, fish, blood, placenta, liver, 

 spleen, kidneys, brain, and vege- 

 table materials, such as yeast, 

 wheat, etc., may serve to furnish 

 the extract used as basis for the 

 medium. 



(2) Add 500.0 g. to 100.0 cc. distilled 

 water or clear tap water. 



(3) Heat the mixture 20 minutes over 

 a free flame at a temperature not 

 exceeding 50°C. 



Note: Or simply keep in a 

 cool place over night. 



(4) Skim off fat floating on the sur- 

 face. 



(5) Raise the temperature to boiling 

 point. 



(6) Boil 10 minutes. 



(7) Pour the mixture on to a wet, 

 thick, clean cloth. 



