CULTURE MEDIA FOR CULTIVATIOX OF MICROORGANISMS 



237 



(11) Filter thru cotton and filter paper 

 in a glass funnel. 



(12) Sterilize in tubes or flasks by- 

 heating in an autoclave for an 

 hour at 15 pounds pressure. 



(nn) Kreidler(1926). 



(1) Infuse 500.0 g. of finely chopped 

 lean beef in 1000.0 cc. water in an 

 ice box over night. 



(2) Boil for 30 minutes over a free 

 flame. 



(3) Filter thru gauze and then thru 

 paper to remove the fat. 



(4) Make up to 1000.0 cc. volume. 



(5) Add 10.0 g. peptone (Difco) and 

 5.0 g. NaCl. 



(6) Boil to dissolve. 



(7) Adjust to pH = 7.8. 



(8) Filter thru paper. 



(9) Sterilize in the Arnold using the 

 fractional method. 



References: Dunham (1887 p. 338), Schultz 

 (1891 p. 56), Frothingham (1895 p. 53), 

 Nicolle (1896 p. 333), Stutzer and Hartleb 

 (1897 p. 404), Wurtz (1899 p. 26), Com- 

 mittee A. P. H. A. (1899 p. 77), Migula 

 (1901 p. 19), Thionot and Masselin (1902 

 p. 19), Smith (1902 p. 80), Frost (1903 

 p. 6), Heinemann (1905 p. 27), Committee 

 A. P. H. A. (1905 p. 107), (1909 p. 285), 

 Smith (1905 p. 195), Lohnis (1913 p. 14), 

 Sears (1916 p. 113), Roddy (1917 p. 41), 

 Mcintosh and Smart (1919 p. 724), Per- 

 cival (1920 p. 56), Bezangon (1920 p. 108), 

 Giltner (1921 p. 29), Dopter and Sac- 

 qu6pee (1921 p. 117), Abbott (1921 p. 124), 

 Foster and Randall (1921 p. 151), Randall 

 and Hall (1921 p. 347), Ayers, Rupp and 

 Mudge (1921 p. 258), Harvey (1921-22 

 pp. 67, 68), Heinemann (1922 p. 31), Pit- 

 field (1922 p. 13), Hartley and Hartley 

 (1922 p. 460), Stitt (1923 p. 30), Klimmer 

 (1923 p. 192), Frost (1923 p. 64), Cunning- 

 ham (1924 p. 11), Park, Williams and 

 Krumwiede (1924 p. 96), Kreidler (1926 

 p. 190). 



780. Jensen's Nitrate Infusion Broth 



Constituents: 



1. Water 1000.0 cc. 



2. Meat 500.0 g. 



3. NaCl 5.0 g. 



4. Peptone 10.0 g. 



5. NaNOs 3.0 g. 



Preparation: 



(1) Prepare a meat infusion from 1 and 2 

 (method not given). 



(2) Dissolve 3, 4 and 5 in (1). 



(3) Adjust to a slight alkalinity with 

 soda. 



Sterilization: Method not given. 

 Use: To study denitrification. 

 Variants : 



(a) Percival prepared a similar medium 

 by adding 3.0 g. NaNOa to a liter of 

 meat infusion (See Dunham's Meat 

 Infusion Peptone Solution, variant 

 (u) 634). 



(b) Harvey prepared a similar medium 

 by adding 5.0 g. KXO3 to a liter of 

 meat infusion (see Dunham's Meat 

 Infusion Peptone Solution, variant 

 (bb) 634). 



Reference: Jensen (1898 p. 406), Percival 

 (1920 p. 56), Harvey (1921-22 p. 67). 



781. Davis and Ferry's Nitrate Infusion 



Broth 



Constituents : 



1. Beef infusion solution 500.0 cc. 



2. 2.0% peptone solution 500.0 cc. 



3. NaCl 4.0 g. 



4. K2HPO4 3.0 g. 



5. MgS04 0.4 g. 



6. KNO3 0.2 g. 



Preparation : 



(1) Preparation of beef infusion not 

 given. 



(2) Mix 1 and 2. 



(3) Dissolve 3, 4, 5 and 6 in (1). 



(4) Add enough N/1 NaOH to give final 

 reaction pH = 8.0 to 8.2. 



(5) Steam 15 minutes and check reaction. 



(6) Distribute as desired. 

 Sterilization: Sterilize at 115° for 20 



minutes. 

 Use: Cultivation of Bad. diphtheriae and 



toxin production. Authors reported good 



growth. 

 Reference: Davis and Ferry (1919 p. 236). 



782. LoefHer's Infusion Broth (Roux and 



Rochaix) 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Beef 500.0 g. 



3. NaCl 5.0 g. 



4. Peptone 20.0 to 25.0 g. 



