242 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(4) Add peptone (1.0%) and NaCl 

 (0.5%). 



(5) Titrate to pH = 8.2. 



(6) Autoclave at 15 pounds pressure 

 for 20 minutes. 



(e) Stitt used the medium as a bile sub- 

 stitute. He used 500.0 g. of ground 

 beef liver, 1 liter of water and 1.0% 

 peptone. The procedure was the 

 same as for Stitt's variant of Dun- 

 ham's Infusion Broth (see variant 

 (gg) 665). 

 References: Cesaris-Demel (1899 p. 532), 

 Pfuhl (1907 p. 379), Kessler (1911 p. 604), 

 Harvey (1921-22 p. 69), Goss (1921 p. 615), 

 Stitt (i924p. 47). 



791. Owen, Martin and Pitts' Trypsin 

 Beef Tea 



Constituents : 



1. Bouillon (Beef tea) 



2. Trypsin 

 Preparation : 



(1) Add 10.0 cc. of trypsin (Liquor pan- 

 creatica. Digestive Ferments Co.) to 

 90.0 cc. of sterile bouillon (Beef tea). 



(2) Distribute in 5.0 to 10.0 cc. lots in 

 sterile test tubes. 



Sterilization: Method of sterilization of 

 beef tea not given. 



Use: Enrichment medium for blood cul- 

 tures. Authors report earlier develop- 

 ment of staphylococci, pneumococci, 

 streptococci and typhoid bacilli than on 

 "glucose beef tea." 



Reference: Owen, Martin and Pitts (1916 

 p. 198). 



792. Kligler's Heart Infusion Broth 



Constituents : 



1. Peptone phosphate solution. 



2. Beef heart. 



3. Saline solution. 

 Preparation : 



(1) Exact composition of peptone phos- 

 phate solution not given. 



(2) Weigh out 2 equal portions of beef 

 heart. 



(3) Suspend one portion of (2) in 10 

 volumes of saline solution and the 

 other in ten volumes of alcohol ether 

 (1:3). The alcohol is added first to 

 desiccate the clots and then the ether. 



(4) Filter the saline extract thru Berke- 



feld candle (length of extraction not 

 given). 



(5) Decant the ether extract, evaporate 

 nearly to dryness under suction, and 

 take up the residue in an amount of 

 saline equal to the original volume. 



(6) Add 1.0 cc. of (4) or (5) or a mixture 

 of the two to 5.0 cc. of (1). 



Sterilization: Method not given. 



Use : To show growth accessory substances 

 for pathogenic bacteria. Author reported 

 that the saline extract contained most 

 growth accessory materials while the 

 ether extracts were practically devoid of 

 them. 



Reference: Kligler (1919 p. 41). 



793. Harvey's Heart Infusion Broth 



Constituents: 1. Ox heart Bouillon. 

 Preparation : 



(1) Prepare bouillon 1% acid to phenol- 

 phthalein from ox heart which has 

 been "hung" 2 days. 



(2) Sow with B. lactis aerogenes. 



(3) Incubate 48 hours. 



(4) Steam 20 minutes. 



(5) Make reaction 1% acid to phenol- 

 phthalein. 



(6) Sow with B. coll. 



(7) Incubate 48 hours. 



(8) Steam 20 minutes. 



(9) Test for absence of sugar by cultiva- 

 tion of B. coll or B. lactis aerogenes, 

 using fermentation tubes. 



(10) Leave in a cool place for the growth 

 to sediment. 



(11) Filter the supernatant fluid thru 

 thick filter paper or thru a porcelain 

 candle. 



(12) Distribute into test tubes. 

 Sterilization : Method not given. 

 Use: Sugar free culture medium. 

 Reference: Harvey (1921-22 p. 113). 



794. Park, Willams and Krumwiede's 

 Heart Infusion Broth 



Constituents: 



1. Water (tap) 1000.0 cc. 



2. Heart (Beef) 500.0 g. 



3. Peptone 15.0 g. 



4. NaCl 5.0 g. 



5. Egg 1 



Preparation : 



(1) Add 500.0 cc. of tap water to 500.0 g. 



