CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



243 



chopped fat and tendon free beef 

 heart. 



(2) Add 15.0 g. of peptone, 5.0 g. NaCI 

 and one well beaten egg to (1). 



(3) Heat in a water bath, double boiler 

 or over the open flame, stirring con- 

 stantly until the color changes to 

 brown (at about 68° to 70°C). 



(4) Strain thru a wire sieve or wire 

 gauze. Do not use cheese cloth, 

 cotton or paper. 



(5) Heat 500.0 cc. of water to 70°C. 



(6) Mix (5) and (4). 



(7) Adjust the reaction to +0.2 phenol- 

 phthalein or to about pH = 7.4. 



(8) Heat in an autoclave at 15 pounds 

 for 30 minutes. 



(9) Remove the kettle carefully and set 

 aside for sedimentation to take place 

 or run in Sharpies centrifuge. 



(10) Decant, if allowed to settle. 



(11) Melt. 



(12) Tube. 



Sterilization: Sterilize in an autoclave at 



15 pounds for 30 minutes. 

 Use: Cultivation of meningococcus. 

 Reference: Park, Williams and Krumwiede 



(1924 p. 126). 



795. Wroblewski's Suprarenal Capsule 

 Infusion Broth 



Constituents : 



1. Water 1000.0 cc. 



2. Suprarenal capsule (ox) 500.0 g. 



3. Peptone 15.0 g. 



4. NaCl 5.0 g. 



5. Soda 

 Preparation : 



(1) Prepare bouillon in the usual manner 

 using suprarenal capsule (ox) instead 

 of fat free beef. 



(2) Dissolve 3, 4 and 5 in (1). 

 Sterilization: Not specified. 

 Use: General culture medium. 

 Reference: Wroblewski (1896 p. 529). 



796. Harvey's Organ Infusion Broth 



Constituents: 



1. Water 1000.0 cc. 



2. Organs (lungs, liver, spleen, 

 testicles, thymus, placenta 



or other organs) 500.0 g. 



3. Peptone 10.0 g. 



4. NaCI 5.0 g. 



Preparation : 



(1) Mince finely lungs, liver, spleen, kid- 

 ney, testicles, thymus, placenta or 

 other organs. 



(2) Proceed as Harvey's Variant of 

 Schultz's Beef Infusion Peptone Solu- 

 tion (see variant (v) 750). 



Reference: Harvey (1921-22 p. 98). 



797. Kligler's Blood Clot Infusion Broth 



Remove all the serum from blood clots 

 by centrifugation and use blood clots in- 

 . stead of beef heart to prepare medium 

 (see 792.) 



798. Hunter's Fish Infusion Broth 



Constituents: 



1. Water 1000.0 cc. 



2. Salt-water trout 1000.0 cc, 



3. Peptone 15.0 g. 



4. Raw fish 

 Preparation : 



(1) Add 1000.0 cc. water and 15.0 g. pep- 

 tone to 1000.0 g. finely chopped salt- 

 water trout or weakfish from which 

 skin and bones have been removed. 



(2) Heat in Arnold or in water both at 

 95°-100°C. for an hour. 



(3) Strain juice thru cheese cloth, filter 

 thru cotton and adjust reaction to 

 neutral. 



(4) Heat in Arnold for 30 minutes at 100° 

 and filter thru paper. 



(5) Tube in 10.0 cc. lots and add to each 

 tube about 1.5 raw fish. 



(6) If anaerobic cultures are desired, 

 cover the surface with layer of liquid 

 petrolatum. 



Sterilization: Sterilize in autoclave for 15 

 minutes at 15 pounds. 



Use : To study the decomposition of fish by 

 organisms resembling colon-aerogenes 

 group. Author reported that some cul- 

 tures produced foul odor and indol. 



Reference: Hunter (1920 p. 543). 



799. Harvey's Fish Infusion Broth 



Constituents : 



1. Water 1000.0 cc. 



2. Fish flesh 500.0 g. 



3. NaCI 26.5 g. 



4. KCI 0.75 g. 



5. Peptone 5.0 g. 



