CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



249 



sterilization: Not specified. 



Use: Water analysis and indol production. 

 Author tested for indol by adding 10.0 

 drops of Ehrlich's reagent and 10.0 drops 

 of a saturated watery solution of po- 

 tassium persulphate to a tube after 

 incubation. 



Variants: Frieber specified the use of 

 Griibler's trypsin and did not give the 

 exact amounts of chloroform or toluol 

 used. 



References: Olszewski and Kohler (1923 

 p. 5), Frieber (1921-22 p. 275). 



811. Bacto Cooledge Broth (Dehydrated) 



Constituents: 



1. Water 



2. Beef extract (Bacto) 3.0 g. 



3. Peptone (Bacto) 10.0 g. 



4. NaCl 5.0 g. 



5. Dibromthymolsulphoneph- 



thalein 0.032 g. 



Preparation : 



(1) Dissolve 18.0 g. of Bacto Cooledge 

 Broth, (dehydrated) in 1000.0 cc. of 

 distilled water. The formula for the 

 broth is given above. 



(2) Reaction pH = 7.0±. 

 Sterilization: Sterilize in the usual manner. 

 Use: General culture medium. 

 Reference: Digestive Ferments Co. (1925 



p. 13). 



812. Heinemann's Bouillon 



Constituents : 



1. Water (tap) 300.0 cc. 



2. Peptone 3.0 g. 



3. Beef extract 1.0 g. 



Preparation : 



(1) Weigh a saucepan and measure 

 300.0 cc. tap water into it. 



(2) Dissolve 3.0 g. of peptone in (1) 

 when hot. 



(3) Make up the loss due to evaporation 

 by adding water. 



(4) Dissolve 1.0 g. of beef extract in (3). 



(5) Adjust the reaction to alkaline to 

 litmus or neutral to phenolphthalein 

 and add 0.5% normal HCl. 



(6) Distribute into Erlenmeyer flasks. 



(7) Autoclave at 120° for 10 minutes. 



(8) Store for 24 hours in a cool place. 



(9) Filter. 

 (10) Tube. 



Sterilization: Autoclave at 120° for 5 



minutes. 

 Use: General culture medium. 

 Variants : 



(a) Committee S. A. B. dissolved 3.0 g. 

 beef extract and 5.0 g. peptone in a 

 liter of distilled water. The medium 

 may be clarified using egg white. 

 Adjust from pH = 6.6 to 7.4. 



(b) Ball, Tanner, Levine (A. P. H. A.) 

 and Park, Williams and Krumwiede 

 used 3.0 g. beef extract and 5.0 g. 

 peptone and adjusted the reaction to 

 + 1, if the reaction does not fall be- 

 tween +0.5 and +1.0. 



(c) Brown used 3.0 or 5.0 g. of Liebig's 

 meat extract and 10.0 or 20.0 g. of 

 Fairchild's peptone or 10.0 g. of 

 Witte's peptone or 10.0 g. of Bacto 

 peptone. Initial pH from 7.1 to 7.4. 



References: Heinemann (1905 p. 25), Com- 

 mittee S. A. B. (1918 p. 115), Ball (1919 

 p. 76), Tanner (1919 p. 44), Levine (1921 

 p. 109), Brown (1921 p. 561), Park, Wil- 

 liams and Krumwiede (1924 p. 131). 



813. Wyant's Bouillon Cube Broth 



Constituents: 



1. Water 1000.0 cc. 



2. Bouillon cubes 3.0 to 5.0 g. 



3. Peptone 10.0 g. 



Preparation : 



(1) Dissolve 2, and 3 in boiling tap water. 



(2) Cool liquid to coagulate fats. 



(3) Filter cold. 



(4) Tube. 



Sterilization: Sterilize at 15 pounds in 

 autoclave. 



Use: Substitute for beef extract or meat in 

 nutrient media. A very satisfactory sub- 

 stitute for meat. Can also be used in 

 special media omitting the peptone. 



Reference: Wyant (1920 p. 189). 



814. Heinemann's Nitrate Broth 



Constituents : 



1. Bouillon 1000.0 cc. 



2. KNO3 5.0 g. 



Preparation: (1) Dissolve 2 in 1. 

 Sterilization: Not specified. 



Use: To study denitrification. 

 Variants : 



(a) Sewerin used 3.0, 6.0, 7.0, 8.0 or 



