CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



251 



816. Thoinot's Arsenious Acid Bouillon 



Constituents : 



1. Bouillon 1000.0 cc. 



2. Arsenious acid 0.1 g. 



Preparation : 



(1) Prepare peptone bouillon. 



(2) Add 0.01% arsenious acid to (1). 

 Sterilization: Not specified. 



Use : Differentiation between colon bacillus 

 and the Eberth bacillus. Author re- 

 ported that the Eberth bacillus did not 

 grow in a medium containing 0.01% 

 arsenious acid. Coli bacillus grew well 

 even in a medium containing 1.5% ar- 

 senious acid. 



Reference: Thoinot (1898 p. 126). 



817. Omeliansky's Dilute Bouillon 

 Constituents : 



1. Distilled water 50.0 cc. 



2. Bouillon 50.0 cc. 



3. Gypsum 5.0 g. 



Preparation : 



(1) Method of preparation of bouillon 

 not given. 



(2) Dilute 50.0 cc. alkaline bouillon with 

 an equal volume of distilled water. 



(3) Add 5.0 g. gypsum to (2). 

 Sterilization: Not specified. 



Use: To study nitrification. The author 

 reported that using a mixture of Bac. 

 ramosus, Nitrosomonas and Nitrobacter, 

 ammonia, nitrites and nitrates were 

 formed. Using Bac. ramosus and Nilro- 

 somonas, ammonia and nitrites were 

 formed. Using Bac. ramosus and Nitro- 

 bacter only ammonia was formed. Using 

 Nitrosomonas and Nitrobacter there was 

 only organic nitrogen present. 



Variant: Omeliansky omitted the gypsum 

 and added 10.0 cc. alkaline bouillon to 

 40.0 cc. distilled water. 



Reference: Omeliansky (1899 p. 487). 



818. Muller and Malvoz's Iodine Bouillon 



Constituents: 



1. Bouillon 1000.0 cc. 



2. CaC03 50.0 g. 



3. Sodium hyposulphite. 



4. KI. 



5. Iodine. 

 Preparation : 



(1) Add 5 parts pure CaCOa to each 100 



parts of ordinary bouillon. Method 

 of preparation of bouillon not given. 

 Mix well. 



(2) Distribute in 90.0 cc. lots in 150 or 

 200.0 cc. flasks. 



(3) Make 50.0 g. of crystalline sodium 

 hyposulphite up to 100.0 cc. by the 

 addition of water. 



(4) Mix thoroly in a mortar 20.0 g. KI 

 and 25.0 g. iodine, and add enough 

 water to make 100.0 cc. 



(5) To each 90.0 cc. of sterile (2) add 

 10.0 cc. of sterile (3) and 2.0 cc. of 

 sterile (4). Mix well. 



(6) Distribute in tubes or flasks. 

 Sterilization: Sterilize (2) and (3) at 100° 



for 30 minutes. Sterilize (4) by itself 



(method not given). 

 Use: Enrichment medium for typhoid 



group. B. coll and other forms are 



inhibited. 

 Reference: Muller and Malvoz (1923 p. 



435). 



819. Fremlin's Ammonium Sulphate 

 Bouillon 



Constituents : 



1. Water 1000.0 cc. 



2. (NH4)2S04 1.0 g. 



3. Potassium phos- 

 phate 1.0 g. 



4. MgCOs 10.0 g. 



5. Peptone beef broth. 0.1 to 100.0 cc. 

 Preparation : 



(1) Dissolve 2 and 3 in part of 1. 



(2) Dissolve 4 in the remainder of 1. 



(3) Mix sterile (1) and sterile (2) and add 

 various amounts of sterile 5. 



Sterilization: Method not specified. 



Use: To study nitrogen oxidation by 



nitroso bacteria. 

 Reference: Fremlin (1903 p. 368). 



820. Stitt's Carbonate Bouillon 



Constituents: 



1. Bouillon. 



2. CaC03. 



Preparation: (1) Add small fragments of 



marble (CaCOs) to bouillon. 

 Sterilization: Not specified. 

 Use: General culture medium. 

 Reference: Stitt (1921 p. 35). 



