254 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



pressure. Sterilize (19) for two hours, 



method not given. 

 Use: Cultivation of tubercle bacilli and 



preparation of tuberculin. 

 Reference: Reeser (1908 p. 152). 



826. Mellon's Serum Veal Infusion Broth 



Constituents: 



1. Veal Infusion Broth 1000.0 cc. 



2. Glucose (1.0%) 10.0 g. 



3. Serum (human or rabbit). 

 Preparation : 



(1) Prepare a 1.0% glucose veal infusion 

 broth. 



(2) Adjust (1) to +1.6 to phenol- 

 phthalein. 



(3) Add a few drops of sterile human or 

 rabbit serum to each tube of (2). 



Sterilization: Not specified. 

 Use: Cultivation of diphtheroids. 

 Reference: Mellon (1916 p. 84). 



827. Albert and Kelman's Blood Veal 

 Infusion Broth 



Constituents : 



1. Veal Infusion Broth 1000.0 cc. 



2. Blood, rabbit defibrinated. . 100.0 cc. 

 Preparation : 



(1) Prepare 1 liter of veal infusion broth. 



(2) Adjust to neutral to phenolphthalein. 



(3) Distribute in 50.0 cc. lots in Erlen- 

 meyer flasks. 



(4) Add 5.0 cc. of sterile defibrinated rab- 

 bit blood to each flask. 



(5) Heat the mixture over a water bath 

 at 75°C. until the blood coagulates 

 and settles on standing (3 to 5 minutes 

 after reaching the temperature). 



Sterilization: Method not given. 



Use : Cultivation of B. infliienzae and toxin 



production. After incubation at 37°C. 



for 24 hours, centrifuge to remove the 



coarse particles and then filter thru a 



mandler candle filter. 

 Reference: Albert and Kelman (1919 p. 



434). 



828. Thjotta and Gundersen's Blood Veal 

 Infusion Solution 



Constituents: 



1. Veal broth. 



2. Sodium phosphate 

 (NajHPOO. 



3. Blood. 



(secondary) 



Preparation : 



(1) Prepare veal broth but use 0.2% 

 NaaHPOi instead of NaCl. 



(2) Adjust to pH = 7.8. 



(3) Add 10.0 cc. of blood to 100.0 cc. of 

 sterile (2). 



(4) Incubate at 37°C. 



Sterilization: Sterilize (2) at 115° for 



30 minutes. 

 Use: Isolation of a streptothrix from the 



blood. 

 Reference: Thjotta and Gundersen (1925 



p. 1). 



829. Robinson and Header's Liver Veal 

 Infusion Broth 



Constituents : 



1. Water 1000.0 cc. 



2. Lean veal 500.0 g. 



3. Peptone 20.0 g. 



4. NaCl 5.0 g. 



5. Liver (Fresh sterile guinea pig). 

 Preparation : 



(1) Mince lean veal fine, and soak in 

 water in ice box for 18 to 24 hours. 



(2) Filter thru wire gauze and heat to 

 80° for 5 minutes. 



(3) Cool and remove fat from the sur- 

 face. 



(4) Add 3 and 4 to (3). 



(5) Adjust to 0.3% acid to phenol- 

 phthalein. 



(6) Boil 5 minutes and adjust again if 

 necessary. 



(7) Filter thru wire gauze and sediment 

 allowed to settle. 



(8) Distribute in 125.0 cc. lots in 300.0 cc. 

 Florence flasks. 



(9) Kill a large guinea pig by a blow on 

 the head and plunge the guinea pig 

 into a 0.5% lysol solution for a few 

 minutes. 



(10) Open the body cavity aseptically and 

 remove the liver. 



(11) Introduce a piece of liver slightly 

 larger than a 25 cent piece into each 

 flask containing 125.0 cc. sterile 

 medium. 



Sterilization: Sterilize (8) by heating at 15 

 pounds pressure for 15 minutes. 



Use: Cultivation of diphtheria bacilli, and 

 production of toxin by the Park Williams 

 strain ^8. Contamination from liver 

 may be detected in smear preparations 



