CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



255 



at the time of testing the toxin and also 

 by the odor of the broth. 

 Reference: Robinson and Meader (1920 

 p. 107). 



830. Starln and Dack's Casein Digest Veal 

 Infusion Broth 



Constituents : 



1. Veal infusion 1000.0 cc. 



2. Peptone 10.0 g. 



3. NaCl 5.0 g. 



4. Casein digest (Wolf's) 50.0 g. 



Preparation : 



(1) Method of preparation or composi- 

 tion of veal infusion not given. 



(2) Dissolve 2, 3 and 4 in (1). The prepa- 

 ration of Wolf's casein digest was not 

 given by the authors. It was pre- 

 pared according to Kahn's method. 

 See 648. 



(3) Filter to remove any precipitated 

 material. 



(4) Adjust reaction to pH = 7.8 to 8.0 

 Sterilization: Method not given. 



Use: Cultivation of Clostridium botulinum. 

 Reference: Starin and Dack (1923 p. 173). 



831. Bacto Veal Infusion Medium 

 (Dehydrated) 



Constituents : 



1. Distilled water. 



2. Veal infusion 8.0 g. 



3. Peptone, Bacto 10.0 g. 



4. Agar, Bacto 10 g. 



Preparation : 



(1) Dissolve 19.0 g. of Bacto Veal Infu- 

 sion Medium (Dehydrated) in 

 1000.0 cc. of distilled water by boil- 

 ing. (Low agar content according 

 to Hitchens. 



(2) Restore the loss in weight if neces- 

 sary. 



(3) If sterilized at 15 pounds for 20 

 minutes pH = 7.5±. 



Sterilization: Sterilize at 15 pounds for 



20 minutes. 

 Use: General culture medium. 

 Reference: Digestive Ferments Co. (1925 



p. 11). 



832. Bruschettinis' Blood Egg Broth 

 (Klimmer) 



Constituents : 



1. Veal Infusion Broth 1000.0 cc. 



2. Blood, defibrinated, rabbit 



or dog 100.0 cc. 



3. Egg yolk. 



Preparation: (1) Add 50.0 cc. of egg yolk 

 and 100.0 cc. of dog or rabbit defibrinated 

 blood to 1000.0 cc. of veal infusion broth. 



Sterilization: Method not given. 



Use: Cultivation of tubercle bacilli. 



Reference: Klimmer (1923 p. 224). 



833. Jackson and Muer's Glucose Liver 

 Infusion 



Constituents: 



1. Water 1000.0 cc. 



2. Liver, beef 500.0 g. 



3. Peptone (Witte) 10.0 g. 



4. Glucose 10.0 g. 



5. K2HPO4 1.0 g. 



Preparation : 



(1) Chop 2 into small pieces and add 1. 

 Weigh the infusion and container. 



(2) Boil slowly for 2 hours in a double 

 boiler, starting cold and stirring it 

 occasionally. 



(3) Make up the loss in weight by evapo- 

 ration and strain thru a wire strainer. 



(4) To the filtrate add 3, 4 and 5. Weigh 

 the infusion and container. 



(5) After warming this mixture in a 

 double boiler and stirring it for a few 

 minutes to dissolve ingredients, ti- 

 trate with N/20 sodium hydrate, using 

 phenolphthalein as an indicator and 

 neutralize with normal sodium 

 hydrate. 



(6) Boil vigorously for 30 minutes in a 

 double boiler and 5 minutes over a 

 free flame with constant stirring to 

 prevent the caramelization of the 

 dextrose. 



(7) Make up any loss in weight by 

 evaporation and filter thru cotton 

 flannel and filter paper. 



Sterilization : Tube and sterilize in an auto- 

 clave for 15 minutes. 



Use: Presumptive test for gas formers in 

 water. Author reported that this me- 

 dium gave a larger amount of attenuated 

 forms, had greater rejuvinating power, 

 gave fewer abnormalities and greater and 

 more rapid gas production than did the 

 usual dextrose broth. B. coli produced 

 gas. 



