CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



steam for 2 hours. Sterilize (13) in 

 streaming steam for 10 minutes. 



Use: Diagnosis of typhoid. Enrichment 

 medium for typhoid. Emulsions were 

 made from the patients' feces and 

 smeared, following 13 hours' incubation, 

 on this medium. 



Variants : 



(a) Lubenau used 0.3% caffeine instead 

 of 0.6% and plated on solid medium. 

 (See medium 1749.) 



(b) Abel gave the following method of 

 preparation: 



(1) Chop 500.0 g. of fat free meat and 

 add to a liter of water at 50°C. 



(2) Keep at 50°C. for 30 minutes and 

 boil for 30 to 45 minutes. 



(3) Filter or strain the fluid from the 

 meat. 



(4) Make up the fluid to one liter. 



(5) Add sufficient NaOH so that the 

 reaction is alkaline to phenol- 

 phthalein. 



(6) Sterilize for 10 minutes in the 

 steamer. 



(7) Add 1050.0 cc. of a 1.2% caffeine 

 solution and 14.0 cc. of a 0.1% 

 crystal violet solution (both the 

 caffeine and dye are dissolved in 

 sterile cold water). 



(8) Add the stools directly to this 

 medium. 



(c) Harvey used infusion broth prepared 

 according to variant (bb) 779, ad- 

 justed to permanently alkaline to 

 phenolphthalein and mixed with 

 equal parts of a 1.0% caffeine solu- 

 tion. No crystal violet was em- 

 ployed. 



References: Ficker and Hoffmann (1904 

 p. 255), Lubenau (1907 p. 248), Abel (1912 

 p. 131), Harvey (1921-22 p. 92), Klimmer 

 (1923 p. 216). 



840. Ayers and Rupp's Hippurate Infusion 

 Broth 



Constituents : 



1. Meat infusion 1000.0 cc. 



2. Peptone (Park Davis) 10.0 g. 



3. Sodium hippurate 10.0 g. 



4. Glucose 2.0 g. 



5. K2HPO4 1.5 g. 



Preparation : 



(1) Method of preparation of meat infu- 

 sion not given. 



(2) Dissolve 2, 3, 4 and 5 in (1). 



(3) Adjust the reaction to pH = 7.2. 

 Sterilization: Method not given. 



Use: To show hydrolysis of sodium hippu- 

 rate by haemolytic streptococci. When 

 more glucose was added, and phosphate 

 omitted, allowing the hydrogen ion con- 

 centration to increase, the streptococci 

 continued to hydrolyse the sodium hip- 

 purate. The development of acidity had 

 little effect. 



Variants: The authors used 2.0 to 5.0 g. 

 glucose with or without 10.0 g. of K2HPO4. 



Reference: Ayers and Rupp (1922 p. 391). 



841. MacConkey's Bile Salt Infusion Broth 

 (Heinemann) 



Constituents: 



1. Infusion broth 100.0 cc. 



2. Sodium taurocholate (0.5%). 0.5 g. 



3. Peptone (2.0%) 2.0 g. 



4. Glucose (0.5%) 0.5 g. 



5. Litmus. 

 Preparation : 



(1) Prepare infusion broth. 



(2) Dissolve 2, 3 and 4 in (1) by heating, 



(3) Filter. 



(4) Add sufficient litmus solution to 

 color. 



Sterilization: Method not given. 



Use: Culture medium used in water 



analysis. 

 Reference: Heinemann (1905 p. 129). 



841a. Smith's Glucose Infusion Broth 



Constituents : 



Infusion broth 1000.0 cc. 



Peptone, Witte 20.0 g. 



NaCl 5.0 g. 



Glucose 1 .0 g. 



Preparation : 



(1) Prepare infusion from beef in the 

 usual manner. 



(2) Add Na2C03, normal solution, to 

 adjust to 1.5 to 2.0% acid. 



(3) Heat to 40°C. and inoculate with 

 30-50.0 cc. of a 12 to 14 hour bouillon 

 culture of B. coli and incubate for 

 16 hours or over night. 



(4) Mix with the white of egg, one egg 

 per liter. 



(5) Boil in a water bath or in an Arnold 

 sterilizer for 45 to 60 minutes. 



Cool and add 2.0% Witte peptone, 



