CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



263 



tension was reduced to from 40 to 41 dynes 

 the growth of streptococci was prevented. 

 Reference: Ayers, Rupp and Johnson (1923 

 p. 204). 



846. Ayers, Rupp and Mudge's Glucose 

 Infusion Broth 



Constituents : 



1. Meat infusion 1000.0 cc. 



2. Peptone (Bacto) 10.0 g. 



3. K2HPO4 10.0 g. 



4. Glucose 10.0 g. 



Preparation : 



(1) Prepare 1000.0 cc. meat infusion. 



(2) Add 2, 3 and 4 to (1). 



(3) Adjust to pH = 7.5. 



(4) Tube in 15 cc. lots in Eldredge tubes. 

 Sterilization: Method not given. 



Use: To study fermentation of glucose by 



streptococci. 

 Reference: Ayers, Rupp and Mudge (1921 



p. 258). 



847. Torrey's Brilliant Green Infusion 

 Broth 



Constituents : 



1. Meat infusion broth 1000.0 cc. 



2. Glucose 10.0 g. 



3. Brilliant green (1.0% aq. 



soln.) 15.0 cc. 



Preparation : 



(1) Prepare meat infusion broth in the 

 usual manner. 



(2) Neutralize to phenolphthalein. The 

 final reaction must be neutral. 



(3) Dissolve 10.0 g. glucose in (2). 



(4) Tube in e.xactly 10.0 cc. quantities. 



(5) Prepare a 1.0% solution of Griibler's 

 brilliant green in distilled water. 



(6) Add 0.15 cc. of (5) to each sterile tube 

 of (4). 



Sterilization: Sterilize in the Arnold 

 steamer. Time not specified. 



Use: Enrichment of the paratyphoid- 

 enteritidis group. Author reported that 

 other dominant fecal bacteria were in- 

 hibited by the dye. 



Reference: Torrey (1913 p. 265). 



848. Torrey's Acetic Acid Infusion Broth 



Constituents: 



1. Meat infusion broth 1000.0 cc. 



2. Glucose 10.0 g. 



3. Acetic acid. 



Preparation : 



(1) Prepare meat infusion broth. 



(2) Add 10.0 g. glucose to 1. 



(3) Add acetic acid to (2) until the reac- 

 tion is 0.2, 0.1 or 0.05 normal acetic 

 acid, using phenolphthalein as an 

 indicator. 



(4) Tube in 10.0 cc. lots. 



(5) Plug with paraffined cotton. 

 Sterilization: Method not given. 

 Use: To study fecal flora. 

 Reference: Torrey (1915 p. 74). 



849. Sherman's Lactose Infusion Broth 



Constituents : 



1. Beef infusion 1000.0 cc. 



2. Peptone 30.0 g. 



3. Na2HP04 10.0 g. 



4. KH0PO4 5.0 g. 



5. Lactose 20.0 g. 



Preparation : 



(1) Prepare 1000.0 cc. beef infusion. 



(2) Dissolve 2, 3, 4 and 5 in (1). 

 Sterilization: Method not specified. 



Use: Show CO2 production in highly 

 buffered medium by Streptococcus kefir, 

 Author reported that in this buffered 

 solution CO2 production approached that 

 in digested milk. 



Reference: Sherman (1921 p. 130). 



850. Harvey's Starch Infusion Broth 



Constituents: 



1. Water 1000.0 cc. 



2. Beef 500.0 g. 



3. NaCl 10.0 g. 



4. Starch (Corn flour) 10.0 g. 



5. Peptone 10.0 g. 



6. Litmus solution. 

 Preparation : 



(1) Mince finely fat-free beef. 



Note: Veal, chicken, ox or horse 

 heart, horse flesh, rabbit flesh, fish, 

 blood, placenta, liver, spleen, kid- 

 neys, brain and vegetable materials, 

 such as yeast, wheat, etc., may serve 

 to furnish the extract used as basis 

 for medium. 



(2) Add 500.0 g. to 1000.0 cc. distilled 

 water or clear tap water. 



(3) Heat the mixture 20 minutes over a 

 free flame, at a temperature not ex- 

 ceeding 50°C. 



Note: Or simply keep in a cool 

 place over night. 



