CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



265 



obtained add 25.0 g. Witte's peptone, 

 15.0 g. NaCl and 30.0 g. mannite. 



(4) Boil for 1.5 hours over a flame. 



(5) Neutralize by the addition of soda. 



(6) Filter. 



(7) Dissolve 0.1 g. neutral red in 100.0 g. 

 distilled water. 



(8) When ready for use add 3.0 cc. of 

 sterile (7) to each 150.0 cc. of the 

 medium. 



(9) Distribute in 15 to 20.0 cc. lots in 

 fermentation tubes. 



Sterilization: Sterilize (6) in streaming 

 steam. Sterilize (7) method not given. 



Use: Detection of Bacterium coli in water. 

 When desired for use, add 100.0 cc. of the 

 water being investigated to 50.0 cc. of 

 sterile (6). Then add 3.0 cc. of neutral 

 red solution as indicated in step (8). 

 The author reported that after 12 to 24 

 hours incubation at 46°C. that the pres- 

 ence of Bad. coli was indicated by a 

 uniform turbidity in the fermentation 

 tube, the formation of gas, and a change 

 in color from a red to a yellow-greenish 

 fluorescence. 



Variants: Klimmer added 25.0 g. Witte's 

 peptone, 15.0 g. NaCl and 30.0 g. man- 

 nitol in a liter of meat infusion, boiled 

 1.5 hours, neutralized, tubed and steril- 

 ized in streaming steam for 2 hours. The 

 addition of neutral red or test water not 

 specified. 



References: Bulir (1907 p. 11), Klimmer 

 (1923 p. 216). 



854. Harvey's Ferric Tartrate Infusion 

 Broth 



Constituents: 



1. Water 1000.0 cc. 



2. Beef 500.0 g. 



3. Peptone 10.0 g. 



4. NaCl 5.0 g. 



5. Ferric tartrate 1.0 g. 



Preparation : 



(1) Prepare infusion broth according to 

 Harvey's method, see variant (bb) 

 665. 



(2) Add 1.0 g. of ferric tartrate to a liter 

 of (1). 



Sterilization: Not specified. 

 Use: General culture medium. 

 Reference: Harvey (1921-22 p. 107). 



855. Harvey's Lead Acetate Infusion 

 Broth 

 Constituents : 



1. Water 1000.0 cc. 



2. Peptone 10.0 g. 



3. Beef 500.0 g. 



4. NaCl 5.0 g. 



5. Lead acetate 1.0 g. 



Preparation : 



(1) Prepare meat infusion broth accord- 

 ing to Harvey's method, see variant 

 (bb) 665. 



(2) Add 1.0 g. of lead acetate to (1). 

 Sterilization: Not specified. 



Use: General culture medium. 

 Reference: Harvey (1921-22 p. 107). 



856. Omeliansky's Formate Infusion Broth 



Constituents : 



1. Meat infusion broth 1000.0 cc. 



2. Sodium formate 5.0 g. 



3. Phenolphthalein drops 



Preparation : 



(1) Prepare meat infusion broth from 

 meat and peptone. 



(2) Add 0.5% sodium formate to (1). 



(3) Add several drops of phenolphthalein. 

 Sterilization: Not specified. 



Use: Differentiation of colon typhoid 

 group. Author reported that Bad. typhi 

 abdom. did not decompose the formate. 

 Bad. coli commune decomposed the 

 formate with the production of gas. 

 Bad. paratyphi A and B decomposed the 

 formate with production gas. Bact. 

 dysenteriae and Bac. faecalis alcaligenes 

 did not decompose the formate. Some 

 of the organisms not producing gas may 

 turn the phenolphthalein red due to the 

 decomposition of some albuminous ma- 

 terial to amino acids or other compounds. 



Variants: The author omitted the phenol- 

 phthalein. 



Reference: Omeliansky (1905 p. 674, 1906-07 

 p. 158). 



857. Jordan's Phenol Infusion Broth 

 Constituents: 



1. Infusion broth 900.0 cc, 



2. Phenol (1.0% soln.) 100.0 cc. 



Preparation : 



(1) Prepare infusion broth according to 

 standard methods (see committee 

 A. P. H. A. 1899, 665). 



