CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



267 



Use: Cultivation of Spirochaeta pallida. 

 The authors reported that the value of 

 the medium would be greatly improved 

 if a small quantity of agar be added 

 (0.2%). 



Reference: Weiss and Wilkes-Weiss (1924 

 p. 226). 

 861. Kohman's Brain Infusion Medium 



Constituents : 



1. Meat infusion broth 



2. Brain. 



Preparation: (1) Grind beef brain and add 

 sufficient meat infusion broth to cover. 



Sterilization: Sterilize in the autoclave. 



Use: To cultivate meningococci and to 

 determine the effect of oxygen tension 

 on growth. 



Reference: Kohman (1919 p. 577). 



862. Kreidler's Glucose Brain Broth 



Constituents : 



1. Water 1000.0 cc. 



2. Beef 500.0 g. 



3. Peptone (Difco) 10.0 g. 



4. NaCl 5.0 g. 



5. Glucose (1.0%) 10.0 g. 



6. Brain. 

 Preparation : 



(1) Infuse 500.0 g. of finely chopped lean 

 beef in 1000.0 cc. water in an ice box 

 over night. 



(2) Boil 30 minutes over a free flame. 



(3) Filter through gauze and then 

 through paper to remove the fat. 



(4) Make up to 1000.0 cc. volume. 



(5) Add 10.0 g. peptone (Difco) and 

 5.0 g. NaCl. 



(6) Boil to dissolve. 



(7) Adjust to pH = 7.8. 



(8) Filter through paper. 



(9) Add 10.0 g. glucose. 



(10) Fill long tubes and add several pieces 

 of calf brain, one cubic centimeter in 

 size, washed in running water. 

 Sterilization: Autoclave at 12 pounds pres- 

 sure for 20 minutes. 

 Use: To study the bacteriology of endo- 

 carditis. 

 Reference: Kreidler (1926 p. 190). 



863. Kligler's Heart Infusion Solutions 



Constituents : 



1. Meat infusion broth. 



2. Saline solution. 



3. Heart (beef). 

 Preparation : 



(1) Chop beef heart into small pieces. 



(2) Divide (1) into six equal portions. 



(3) Take up each portion of (2) with 

 9 volumes of saline solution. 



(4) Treat each one of the suspensions in 

 one of the following ways: 



(a) Keep in the ice box over night and 

 steam in the Arnold sterilizer for 

 an hour. 



(b) Keep in the ice box over night and 

 filter thru a Berkefeld candle. 



(c) Keep at 55°C. over night and heat 

 in the Arnold sterilizer for an hour. 



(d) Keep at 55°C. over night and filter 

 thru a Berkefeld candle. 



(e) Extract by boiling one hour and 

 then steam for an hour in the 

 Arnold. 



(f) Extract by boiling for one hour. 



(5) Take 1.0 cc. of one of (4) and add to 

 sterile meat infusion broth. 



Sterilization: Method not given. 



Use: To study the effect of growth stimu- 

 lating materials on the growth of patho- 

 genic bacteria. Author reported that 

 heat destroyed the growth stimulating 

 materials. 



Reference: Kligler (1919 p. 43). 



864. Havens and Taylor's Kidney and Blood 

 Infusion Broth 



Constituents : 



1. Meat infusion 1000.0 cc. 



2. Peptone (1.0%) 10.0 g. 



3. Na2HP04 (1.0%) 10.0 g. 



4. Glucose (0.5%) 5.0 g. 



5. Kidney (rabbit). 



6. Blood, defibrinated, (sheep or rabbit). 

 Preparation : 



(1) Add 2, 3 and 4 to 1000.0 cc. of ordinary 

 meat infusion made with distilled 

 water. 



(2) Adjust to pH 8.0 to 8.2. 



(3) Tube in 10.0 cc. quantities. 



(4) Add a fragment of sterile rabbit 

 kidney and 1.0 cc. of defibrinated 

 sheep or rabbit blood to each tube 

 of sterile (3). 



Sterilization: Sterilize (3) in the autoclave 

 at 10 pounds pressure for 10 minutes. 



