268 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Use: Cultivation of hemolytic strepto- 

 cocci. 



Reference: Havens and Taylor (1921 

 p. 313). 



865. Robertson's Cooked Meat Medium 

 (Torrey) 



Constituents : 



1. Water 1000.0 cc. 



2. Beef heart 500.0 g. 



3. Peptone 10.0 g. 



Preparation : 



(1) Mix 500.0 g. finely minced fresh beef 

 heart with 10.0 g. peptone and a liter 

 of water. 



(2) Cook in a double boiler with just 

 enough heat to cause a slight simmer- 

 ing for about 10 minutes. 



(3) Adjust to pH = 7.2. 



(4) Continue to cook for 1| hours. 



(5) Readjust the reaction if necessary. 



(6) Decant the broth. 



(7) Place the meat in test tubes to a 

 height of 5 cm. 



(8) Add 5.0 cc. of sterile broth to each 

 sterile tube of (7). 



Sterilization: Sterilize flasks of (6) and 

 tubes of (7) separately in the autoclave 

 at 15 pounds pressure for 15 minutes. 

 Following mixing, place the tubes in the 

 Arnold sterilizer on each of two successive 

 days for 20 minutes. 



Use: Analysis of fecal flora. 



Reference: Torrey (1926 p. 355). 



866. Wade and Manalang's Blood Infusion 

 Broth 



Constituents : 



1. Distilled water. 



2. Meat infusion broth. 



3. Blood. 

 Preparation : 



(1) The beef infusion broth contains 

 Witte's peptone and NaCl in various 

 concentrations. Exact amounts not 

 given. 



(2) Thoroly lake sheep or horse blood by 

 adding 20.0 cc. blood to 100.0 cc. dis- 

 tilled water. 



(3) Heat to 80 to 85°C. 



(4) Precipitate the proteins while hot by 

 adding strong hydrochloric acid. 



(5) Filter first thru gauze, then thru 

 paper. 



(6) Reduce the filtrate to 1.0% acid. 



(7) Mix equal parts of sterile (6) and 

 sterile (1). 



Sterilization: Method of sterilization of (1) 

 not given. Sterilize (6) by repeated 

 heating at 65°C. or by filtering. 



Use: Cultivation of Bacillus influenzae, 

 The author reported that better growth 

 was obtained using double strength 

 broth. 



Variants: Author mixed (6) with equal 

 parts of double strength broth. 



Reference: Wade and Manalang (1920 

 p. 98). 



867. Harvey's Ascitic Fluid Infusion Broth 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone 10.0 g. 



3. Beef 500.0 g. 



4. NaCl 5.0 g. 



5. Ascitic fluid 500.0 cc. 



Preparation : 



(1) Prepare meat infusion broth accord- 

 ing to Harvey's method, (see variant 

 (bb) 665). 



(2) Mix 2 parts (1) with one part ascitic 

 fluid. 



(3) Test sterility before use by incuba- 

 tion 48 hours. 



Sterilization: Sterilize in the water bath 

 30 minutes at 56°C., on each of five suc- 

 cessive days. 



Use: Cultivation of parasitic and saphro- 

 phytic bacteria. 



Reference: Harvey (1921-22 p. 83). 



868. Stryker's Serum Infusion Broth 



Constituents : 



1. Meat infusion broth 900.0 cc. 



2. Serum 100.0 cc. 



Preparation : 



(1) Prepare meat infusion broth. 



(2) Mix 1 part highly potent anti-pneu- 

 mococcus horse serum with 9 parts 

 normal horse serum. 



(3) Mix 100.0 cc. of (2) with 900.0 cc. 

 of (1). 



Sterilization: Method not specified. 



Use: To study variation in pneumococcus. 



Other investigators used similar media 



for different purposes. 

 Variants : 



(a) The author used plain serum instead 



