CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



271 



(3) Filter. 



(4) To one liter of (3) add 500.0 g. finely 

 chopped meat. 



(5) Allow to stand for 24 hours. 



(6) Strain. 



(7) Add and dissolve 4 and 5 in (6). 



(8) Boil to precipitate albuminous ma- 

 terials. 



(9) Filter. 



(10) Add 4.0% glycerin. 



(11) Neutralize (may be used without 

 neutralization). 



Sterilization: Not specified. 



Use: Cultivation of tubercle bacilli. 



Reference: Lubinski (1895 p. 126). 



874. Jurewitsch's Potato Infusion Broth 



Constituents: 



1. Water 1500.0 to 2000.0 cc. 



2. Potato 500.0 g. 



3. Meat 500.0 g. 



4. Peptone (Witte or 

 Chapoteaut) 0.5% 



5. NaCl 0.25% 



6. Glycerol 3.0% 



Preparation : 



(1) Peeled potatoes are cut into large 

 pieces, washed off with water and 

 pulverized. 



(2) Weigh the potato pulp and add an 

 equal weight or double weight of 

 water depending on the type of 

 potato. If the potato contains lots 

 of juice do not add so much water. 



(3) Allow (2) to infuse in the cold for 

 one day, shake and strain thru a 

 straining cloth. 



(4) After -\ to i hour pour the liquid 

 from the sediment. 



(5) During this time prepare a meat 

 infusion using 500.0 g. of finely 

 chopped meat to 1000.0 g. of water. 

 Press thru a straining cloth after 

 24 hours. 



(6) Mix equal parts of (5) and (4). 



(7) Add 0.5% Chapoteaut's or Witte'a 

 peptone and 0.25% NaCl. 



(8) Warm gently until peptone is com- 

 pletely dissolved. 



(9) Boil in a Koch steamer for 1 hour 

 and filter warm thru a folded filter. 

 It may be necessary to allow the 

 mixture to settle and pour off the 

 clear liquid and filter that. 



(10) Add 3.0% glycerin and add a satu- 

 rated solution of sodium carbonate 

 until the reaction is decidedly 

 alkaline. 



(11) Boil in the autoclave for i to i hour 

 at 118-120°C., cool and filter. 



(12) Distribute as desired. 

 Sterilization: Sterilize at 115°C. for ^ to 



1 hour. 



Use: Cultivation of tubercle bacilli. Me- 

 dium should be dark brown in color. 



Variants : 



(a) The author used 0.25% HK2PO4 

 instead of NaCl. 



(b) Kolle and Wesserman gave the fol- 

 lowing method of preparation: 



(1) Prepare a pulp from clean peeled 

 potatoes. 



(2) Add 1000.0 cc. of water to 500.0 g. 

 of (1) and allow to stand for one 

 day in the cold. 



(3) Shake thoroughly and filter 

 through linen. 



(4) Allow to settle for 15 to 30 minutes 

 and decant the clear water. 



(5) Soak 500.0 g. of lean meat in 

 1000.0 cc. of water for 24 hours. 



(6) Press thru linen. 



(7) Mix equal parts of (4) and (6). 



(8) Add 1% peptone and 0.5% NaCl 

 and boil for one hour. 



(9) Filter. 



(10) Add 3% glycerol and make dis- 

 tinctly alkaline by the addition 

 of a saturated soda solution. 



(11) Autoclave for 15 to 30 minutes. 



(12) Filter. 



(13) Sterilize once more. 

 Reference: Jurewitsch (1908 p. 664), Kolle 



and Wasserman (1912 p. 409). 



875. Olitsky and Gates' Bacteria Infusion 

 Broth 



Constituents : 



1. Meat infusion broth 1000.0 cc. 



2. Glucose 10.0 g. 



3. B. coll communis 

 Preparation : 



(1) Prepare meat infusion broth with 

 1.0% peptone. 



(2) Add 1.0% dextrose to (1). 



(3) Distribute in 100.0 cc. lots. 



(4) Seed with a broth culture of B. coli 

 communis. 



