CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



273 



a mandler or allow to sediment and 

 pour off the supernatant fluid. 

 (10) Add 75.0 cc. of (9) to flasks contain- 

 ing 1500.0 cc. of beef infusion. 

 Sterilization: Final sterilization not 



specified. 

 Use: Cultivation of influenza bacilli, pneu- 



mococci and streptococci. 

 Reference: Kitchens (1922 p. 35). 



879. Kitchens' Semisolid Glucose Agar 



(Mulsow) 



Constituents: 



1. Water 1000.0 cc. 



2. Beef 1.0 pound 



3. Peptone 20.0 g. 



4. KNO3 2.0 g. 



5. Agar (0.2%) 1.0 g. 



6. Glucose 2.0 g. 



Preparation : 



(1) Add 500.0 cc. of water to 1 pound of 

 ground lean beef, and allow to stand 

 at 37°C. for 48 hours. 



(2) Express the juice and add 20.0 g. pep- 

 tone, 2.0 g. KNO3 and an equal 

 amount (500.0 cc.) of a 0.2% agar 

 solution heated and cooled to 60°C. 



(3) Adjust the reaction to +0.9 to phenol- 

 phthalein. 



(4) Heat in the autoclave at 15 pounds 

 pressure for 25 minutes. 



(5) Filter and readjust the reaction if 

 necessary. 



(6) Add 2.0 g. glucose. 

 Sterilization: Final sterilization not spec- 

 ified. 



Use: Cultivation of gonococcus. Mulsow 

 reported that the gonococcus grew as well 

 if not better than any contaminating 

 organism during the first 12-18 hours. 



Reference: Mulsow (1925 p. 423). 



880. Menten and Manning's Lactose Bile 

 Infusion Solution 



Constituents : 



1. Infusion broth 100.0 cc. 



2. Bile, fresh ox 900.0 cc. 



3. Peptone 10.0 g. 



4. Lactose 10.0 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 



Use: Enrichment of enteritidis-paraty- 

 phoid B organisms from animal tissue. 



Reference: Menten and Manning (1925 

 p. 401). 



881. Kitchen's Glucose Agar Infusion 

 Solution (Mulsow) 



Constituents: 



1. Water 1000.0 cc. 



2. Beef 1.0 1b. 



3. Peptone 20.0 g. 



4. KNO3 2.0 g. 



5. Agar 1.0 g. 



6. Glucose 2.0 g. 



Preparation : 



(1) Grind one pound of lean beef and 

 infuse in 500.0 cc. of water at 37°C. 

 for 48 hours. 



(2) Add 20.0 g. peptone, 2.0 g. KNO3 and 

 an equal amount of an 0.2% agar 

 solution, heated and cooled to 60°C. 

 to the juice from (1). 



(3) Adjust to +0.9 to phenolphthalein. 



(4) Heat in the autoclave at 15 pounds 

 pressure for 25 minutes. 



(5) Filter and adjust the reaction if 

 necessary. 



(6) Add 2.0 g. glucose. 



(7) Final reaction to be between 6.6 and 

 6.8 with an optimum at pH 6.8. 



Sterilization: Final sterilization not 

 specified. 



Use: Isolation of gonococci. The author 

 reported that the gonococci grew as well 

 if not better than the contaminating 

 organism the first 12 to 18 hours. The 

 addition of one part ascitic fluid to four 

 parts agar gave only slightly better 

 results. 



Reference: Mulsow (1925 p. 420). 



882. Kreidler's Trypsinized Broth 



Constituents : 



1. Water 1000.0 cc. 



2. Beef 500.0 g. 



3. Peptone (Difco) 10.0 g. 



4. NaCl 5.0 g. 



5. Trypsin (10% soln.) 10.0 cc. 



Preparation : 



(1) Infuse 500.0 g. finely chopped lean 

 beef in 1000.0 cc. water in an ice box 

 over night. 



(2) Boil 30 minutes over a free flame. 



(3) Filter thru gauze and then thru paper 

 to remove the fat. 



(4) Make up to 1000.0 cc. volume. 



(5) Add 10.0 g. peptone (Difco) and 

 5.0 g. NaCl. 



(6) Boil to dissolve. 



(7) Adjust to pH = 7.8. 



