274 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(8) Filter thru paper. 



(9) To 1000.0 cc. of sterile (8) add 10.0 cc. 

 of a sterile 10% solution of trypsin 

 in normal NaCl solution. 



Sterilization: Sterilize (8) by the frac- 

 tional method in the Arnold. Sterilize 

 the trypsin solution by passing through 

 a porcelain filter. 



Use: To study the bacteriology of endo- 

 carditis. 



Reference: Kreidler (1926 p. 190). 



883. Richardson's Carcinoma Infusion 

 Broth 



Constituents : 



1. Water 1000.0 cc. 



2. Tumor mass 500.0 g. 



S. Peptone 10.0 g. 



4. NaCl 5.0 g. 



5. Glucose 20.0 g. 



6. Tartaric acid 10.0 g. 



Preparation : 



(1) Remove the tumor mass from the 

 breast and strip off as much fat as 

 possible. A few pieces of muscle 

 clear of all fat are also included. 



(2) Weigh this material, chop finely and 

 make up in the same proportions as in 

 ordinary bouillon. (Exact propor- 

 tions not given.) 



(3) Boil thoroly, filter and boil again. 



(4) While in boiling condition add pep- 

 tone and salt as in ordinary bouillon, 

 (Exact amounts not given.) 



(5) Neutralize carefully (method not 

 given), boil again for a short while 

 and while hot add 2.0% glucose and 

 1.0% tartaric acid. 



(6) Cool in a flask — boil again and filter 

 and tube hot. 



Sterilization : Sterilize in the Arnold steril- 

 izer 3 times, one-half hour each time. 

 When a tube is to be inoculated, sterilize 

 for I hour just before inoculation. 



Reference: Richardson (1900-01 p. 72). 



884. Siebert's Horse Meat Infusion Broth 



Constituents: 



1. Water 1000.0 cc. 



2. Horse meat 250.0 g. 



3. Peptone (Witte's) 10.0 g. 



4. NaCl 5.0 g. 



5. Glycerol 37.0 g. 



Preparation : 



(1) Prepare an infusion from 1 part 

 horse meat and 4 parts water. 



(2) Dissolves, 4 and 5 in (1). 



(3) Adjust so that there is 10.0 cc. of N/1 

 alkali per liter using litmus as an 

 indicator. 



(4) Distribute in 40.0 cc. lots in Erlen- 

 meyer flasks. 



Sterilization: Method not given. 

 Use: Cultivation of tubercle bacilli. 

 Reference: Siebert (1909 p. 305). 



885. Peklo's Potato Horse Meat Infusion 

 Broth 



Constituents : 



1. Potato juice 500.0 cc. 



2. Meat (horse infusion) 500.0 cc. 



3. Peptone (Witte's) 5.0 g. 



4. KH2PO4 2.5 g. 



5. Glycerol 30.0 g. 



Preparation : 



(1) Prepare a pulp from raw potatoes. 



(2) Add a little distilled water. 



(3) Allow to stand in the cold for 24 

 hours. 



(4) Press thru a towel. 



(5) Heat until no more coagulum is 

 formed. 



(6) Filter. 



(7) Prepare a meat infusion from horse 

 meat in the same manner as in steps 

 (1) thru (6). 



(8) Mix 500.0 cc. of (6) and (7). 



(9) Dissolve 3, 4 and 5 in (8). 



(10) Add NasCOa so that the reaction will 

 be alkaline after sterilization. 

 Sterilization : Method not given. 

 Use: Cultivation of plant actinomyces. 

 Variants: The author gave the following 



variants: 



(a) Omitted the K2HPO4 and glycerol. 



(b) Omitted the glycerol, used 5.0 g. 

 K2HPO4 instead of 2.5 g. and added 

 1.0 g. K2CO3, and 1.5 g. MgS04. 

 The medium was filtered and steril- 

 ized. 



Reference: Peklo (1910 p. 551). 



886. Hida's Horse Meat Infusion Broth 



Constituents : 



1. Water 1000.0 cc. 



2. Meat, horse 500.0 g. 



3. Burdock root 40.0 g. 



