CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



275 



4. Peptone (Witte) 20.0 g. 



5. NaCl 5.0 g. 



Preparation : 



(1) Boil 500.0 g. finely chopped horse 

 meat and 40.0 g. of finely chopped 

 burdock root in 1 liter of water for 

 two hours. 



(2) Filter. 



(3) Add 20.0 g. Witte's peptone and 5.0 g. 

 NaCl to the bluish filtrate. 



(4) Neutralize by the addition of alkali, 

 using phenolphthalein, or an indi- 

 cator. 



(5) Add 6.0 cc. of a normal alkali solution 

 to (4). 



(6) Boil for another hour. 



(7) Cool and filter. 



(8) Distribute in flasks. 

 Sterilization: Sterilize in the autoclave. 

 Use: Cultivation of diphtheria bacilli and 



toxin production. Author reported that 



0.001 cc. of a 10 day culture at 33°C. 



killed a 250.0 g. guinea pig in 4 days. 



The roots of the compositae generally 



contain from 2 to 3% inulin. 

 Variants: The author substituted Arctium 



lappa compositae for burdock roots. 

 Reference: Hida (1910 p. 412). 



887. Khouvine's Fecal Infusion Broth 



Constituents : 



1. Water 750.0 cc, 



2. Fecal infusion 250.0 cc. 



3. Cellulose 250.0 cc. 



4. CaCOs 2.0 g. 



5. Peptone (pancreatic) 1-0 g. 



6. NaCl 1-0 g. 



7. K2HPO4 1-0 g- 



Preparation : 



(1) Mix one part feces with 8 to 10 parts 

 distilled water. 



(2) Filter on a Laurent filter until clear. 



(3) Allow to settle and decant the clear 

 liquid. 



(4) Mix 250.0 cc. of sterile (4) with 750.0 g. 

 of water and 250.0 cc. of precipitated 

 cellulose that has been dissolved in 

 Schweitzer's reagent. 



(5) Dissolve 4, 5, 6 and 7 in (4). 

 Sterilization: Sterilize the filtrate from (2) 



in the autoclave at 110°C. for 15 minutes. 

 Sterilization of other material or final 

 sterilization not specified. 



Use: Isolation and enrichment of B. cellu- 



lose dissolrens. 

 Reference: Khouvine (1923 p. 715). 



888. Cutler's Blood Clot Infusion Broth 

 (Stitt) 



Constituents: 



1. Water 1000.0 cc. 



2. Blood clot (human) 500.0 cc. 



3. NaCl (0.5%) 5.0 g. 



4. Peptone (1.0%) 10.0 g. 



5. Blood 

 Preparation : 



(1 ) Take 500 .0 cc . of human blood clot and 

 boil for one hour in 1 liter of water. 



(2) Filter. 



(3) Add 1.0% peptone and 0.5% NaCl. 



(4) Tube. 



(5) Add a few drops of blood to each 

 sterile tube just before use. 



Sterilization: Sterilize (4) for 20 minutes 



on each of 3 successive days. 

 Use: Cultivation of Entamobea histolytica 



and other intestinal protozoa or amoeba. 

 References: Cutler (1918 p. 22), Stitt (1923 



p. 51). 



889. Percival's Urea Extract Broth 



Constituents : 



1. Water 1000.0 cc. 



2. Beef extract (Lemco) 50.0 g. 



3. Peptone (Witte) 10.0 g. 



4. Urea 100.0 g. 



Preparation : 



(1) Boil 2, 3 and 4 in 1. 



(2) Cool. 



(3) Medium should be slightly alkaline. 

 If not, add a drop or two of 

 (NH4)2C03 solution. 



(4) Place in a 250.0 cc. flask. 

 Sterilization: Not specified. 



Use: Cultivation of soil forms, Urobacillus 

 Pasteurii. Medium inoculated with gar- 

 den soil. Author reported that at first 

 a stained mount showed Urococci or 

 Planosarcins. After a few days, however, 

 a pure culture of Urobacillus Pasteurii 

 developed. 



Reference: Percival (1920 p. 223). 



890. Stutzer's Dinitro Benzol Extract Broth 



Constituents : 



1. Water 1000.0 cc. 



2. Potassium citrate 10.0 g. 



