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CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



3. Dinitro benzol 2.0 g. 



4. K2HPO4 1.0 g. 



5. Peptone 



6. Meat extract (Schulke and Meyer) 

 Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Reaction to be slightly alkaline. 



(3) Distribute in 10.0 cc. lots. 

 Sterilization: Method not given. 



Use: To study denitrification. Author re- 

 ported no growth using B. agilis, B. nitro- 

 vorus, B. Stutzeri, B. Hartlebi. 



Reference: Stutzer (1901 p. 86). 



891. Harrison and Vanderleck's Aesculin 

 Extract Broth 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Peptone 10.0 g 



3. Liebig's Meat Extract 2.0 g 



4. Iron citrate 2.5 g 



5. Aesculin 1.0 g 



6. Bile salt (Commercial) 5.0 g 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Adjust reaction to +1.0. 

 Sterilization: Not specified. 



Use: Detection of B. coli and B. typhosus. 



B. coli caused a blackening of the medium. 

 Reference: Harrison and Vanderleck (1909 



p. 622). 



892. Sawin's Glycocholate Extract Broth 



Constituents : 



1. Water 1000.0 cc. 



2. Peptone 10.0 g. 



3. Lactose 10.0 g. 



4. Beef extract 7.5 g. 



5. Gelatin 5.0 g. 



6. Sodium glycocholate 2.0 g. 



7. Pancreatin 1.0 g. 



Preparation : 



(1) Heat 2, 3, 4 and 5 in 1 over boiling 

 water or in the steamer for 30 minutes. 



(2) Neutralize in the usual way (method 

 not given). 



(3) Add 2.0 g. of sodium glycocholate dis- 

 solved in a little hot water and 1.0 g. 

 pancreatin to (2). 



Sterilization: Not specified. 



Use: Presumptive test for B. coli. En- 

 richment medium in water analysis. 

 Author reported that best results were ob- 

 tained when pancreatin was present. 

 B. coli produced gas. 



Variants: The author omitted the pan- 

 creatin. 

 Reference: Sawin (1909 p. 388). 



893. Olszewski and Kohler's Trypsinized 

 Bile Salt Extract Broth 



Constituents : 



1. Water 1000.0 cc. 



2. Peptone 10.0 g. 



3. Liebig's meat extract 5.0 g. 



4. NaCl 5.0 g. 



5. Sodium taurocholate (0.1%). 1.0 g. 

 Preparation : 



(1) Prepare ordinary nutrient bouillon 

 using 10.0 g. peptone, 5.0 g. Liebig's 

 meat extract and 5.0 g. NaCl. 



(2) Make alkaline by the addition of 

 7.0 cc. N/1 soda solution after neu- 

 tralizing to litmus. 



(3) Boil. 



(4) Cool to 40°C. and add 0.2 g. trypsin, 

 10.0 cc. chloroform and 5.0 cc. toluol. 



(5) Place in a glass stoppered flask and 

 place in the incubator for 24 to 48 

 hours, shaking occasionally. 



(6) Filter thru a moistened folded filter 

 paper. 



(7) Add 0.1% sodium taurocholate to (6). 

 (Not specified if the salt or gall is to 

 be added before the dilution with 

 physiological salt solution or not.) 



(8) Distribute in 5.0 cc. lots. 

 Sterilization: Not specified. 



Use: Indol production. Dilute one part of 



(6) with 5 parts physiological salt solution 



when desired for use. 

 Variants : Author used 2.0% sterile beef bile 



instead of sodium taurocholate. 

 Reference: Olszewski and Kohler (1923 



p. 6). 



894. Frieber's Tryptophane Extract Broth 



Constituents : 



1. Water 1000.0 cc. 



2. Peptone (1.0%) 10.0 g. 



3. Meat extract (Liebig's 0.5%). 5.0 g. 



4. NaCl (0.5%) 5.0 g. 



5. Tryptophane (0.03%) 0.3 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Neutralize and add 6.0 cc. of normal 

 soda solution per liter. 



(3) Dissolve 0.03% tryptophane in (2). 

 Sterilization: Not specified. 



Use: Indol production. Author reported 



