CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



277 



that Salkowski's test (H2SO4 + nitrites) 

 gave a positive test with materials other 

 than indol. Indol was not formed in the 

 presence of glucose. 



Variants: The author added 3.0% glucose 

 and about 10.0% sterile CaCOa. 



Reference: Frieber (1921-22 p. 265). 



895. Bachmann's Indicator Extract Broth 



Constituents : 



1. Water 1000.0 cc. 



2. Dextrose (0.5%) 5.0 g. 



3. Peptone (0.5%) 5.0 g. 



4. Meat e.xtract (0.5%) 5.0 g. 



5. Indigo carmine (0.04%) 0.4 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1, 



(2) Adjust the reaction to a slightly alka- 

 line reaction by the addition of 

 Na^COs. 



Sterilization: Not specified. 



Use: Cultivation of obligate anaerobes. 

 Other investigators used the medium for 

 various purposes. 



Variants: Rogers, Clark and Davis tested 

 the reduction ability of lactic acid organ- 

 isms using the following solution: 



1. Water 1000.0 cc. 



2. Beef extract 4.0 g. 



3. Peptone 10.0 g. 



4. Glucose 5.0 g. 



5. Neutral red (Griibler's, 

 0.5%soln.) 10.0 cc. 



References: Bachmann (1912-13 p. 7), 

 Rogers, Clark and Davis (1914 p. 415). 



896. Rogers, Clark and Evans' Glucose 

 Extract Broth 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Beef extract (Liebig) 4.0 g. 



3. Peptone (Witte) 10.0 g. 



4. Dextrose (Kahlbaum) 10.0 g. 



5. K2HPO4 5.0 g. 



Preparation : 



(1) Add 2 and 3 to 1, heat on steam bath 

 for 20 minutes. 



(2) Filter and make up loss of water. 



(3) Neutralize with N/1 NaOH using 

 phenolphthalein. 



(4) Heat 5 minutes, filter. 



(5) Test reaction and neutralize again 

 and filter if necessary. 



(6) Dissolve 4 and 5 in (5). 



(7) Make up (6) to 1000.0 cc. 



Sterilization: Method not given. 



Use: To study fermentation and buffer 



effect. 

 References: Rogers, Clark and Evans 



(1914 p. 103), Burton and Rettger (1917 



p. 165). 



897. Waksman and Joffe's Glucose Extract 

 Broth 



Constituents : 



1. Water 1000.0 cc. 



2. Peptone 10.0 g. 



3. NaCl 5.0 g. 



4. Liebig's meat extract 5.0 g. 



5. Glucose 10.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Adjust reaction to pH = 8.0. 



(3) Tube in 10-12 cc. lots. 

 Sterilization: Sterilize at 15 pounds pres- 

 sure for 15 minutes. 



Use : To study change in reaction by actino- 



mycetes metabolism. 

 Reference: Waksman and Joffe (1920 p. 41). 



898. Whipple's Glucose Extract Broth 



Constituents: 



1. Water 1000.0 cc. 



2. Beef extract (Liebig's) 10.0 g. 



3. Peptone 10.0 g. 



4. Dextrose 10.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Adjust the reaction to 0. 



(3) Tube in Smith fermentation tubes. 

 Sterilization: Sterilize in the autoclave. 

 Use: Presumptive test for B.coli. Author 



reported that B. coli produced 25 to 70% 

 gas in the closed arm. Different investi- 

 gators used similar media for various 

 purposes. 

 Variants : 



(a) Henneberg cultivated lactic acid bac- 

 teria in the same medium using 5.0% 

 glucose instead of 1.0%. 



(b) Heinze studied the nitrogen assimi- 

 lation of green algae, Chlorella, 

 Chlorothecium and Stichococcus, and 

 reported that nitrogen was not as- 

 similated. He used a medium con- 

 taining 0.5% meat extract, 0.5% pep- 

 tone and 0.5% glucose per liter of 

 water. 



(c) Buchan studied the ability of organ- 

 isms found in ice cream to give the 



