278 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Voges-Proskauer reaction and used 

 the following medium: 



1. Distilled water 980.0 cc. 



2. Glucose 5.0 g. 



3. Peptone 10.0 g. 



4. Lemco 5.0 g. 



(d) Zikes cultivated, cladothrix, Clado- 



thrix dichotoma and sphaerotilus in 

 the following medium: 



1. Distilled water 1000.0 cc. 



2. Peptone 2.5 g. 



3. Glucose 2.5 g. 



4. Meat extract 0.5 g. 



References: Whipple (1902 p. 424), Henne- 



berg (1903 p. 7), Heinze (1906 p. 647), 

 Buchan (1910 p. 108), Zikes (1915 p. 533). 



899. Johnson's Glucose Phenol Extract 

 Broth 



Constituents : 



1. Distilled water 1100.0 cc. 



2. Beef extract (Liebig's) 10.0 g. 



3. Peptone 10.0 g. 



4. Glucose 10.0 g. 



5. Phenol 5.0 cc. 



6. HCl 4.0 cc. 



Preparation : 



(1) Dissolve 2, 3 and 4 in a liter of dis- 

 tilled water. 



(2) Adjust to neutral to phenolphthalein. 



(3) Dissolve 5.0 g. of phenol and 4.0 cc. 

 of HCl in 100.0 cc. of water. (This 

 is Parietti's solution.) 



(4) Add 20.0 cc. of (3) to (2). 

 Sterilization: Method not given. 



Use: Isolation of Bacillus coli communis 



from alimentary tract of fish. 

 Reference: Johnson (1905 p. 351). 



900. Weisser's Sucrose Extract Broth 



Constituents : 



1. Water 1000.0 cc. 



2. Sucrose (1.0%) 10.0 g. 



3. Peptone (0.1%) 1.0 g. 



4. Meat extract (0.1%) 1.0 g. 



5. Litmus — to color 

 Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Make slightly alkaline by the addi- 

 tion of soda. 



(3) Add litmus until the solution is 

 colored light blue. 



(4) Distribute in Erlenmeyer flasks. 

 Sterilization: Not specified. 



Use: Cultivation of cholera and typhoid 

 group. Other investigators used similar 

 media for different purposes. 



Variants : 



(a) Author used 5.0%, 10.0% or 20.0% 

 sucrose instead of 1.0% and added 

 1.0% normal NaOH solution. 



(b) Author used 20.0% sucrose and 

 added 2.0% normal NaOH solution. 



(c) Author used 10.0% sucrose, 1.0% 

 peptone, 0.5% meat extract, omitted 

 the litmus and added 2.0% normal 

 NaOH solution. 



Reference: Weisser (1886 pp. 335, 339). 



901. Koegel's Lactose Extract Broth 



Constituents : 



1. Water 1000.0 cc. 



2. Peptone 10.0 g. 



3. Lactose 10.0 g. 



4. Meat extract (Liebig's) .... 5.0 g. 

 Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Method not given. 



Use: Cultivation of L. bulgaricus. Other 

 investigators have used similar media for 

 a variety of purposes. 



Variants : 



(a) Obst used a medium containing 1.0% 

 peptone, 1.0% lactose and 0.5% 

 Liebig's meat extract for the pre- 

 sumptive test for B. coli in water 

 analysis. She reported that generally 

 this gave a more delicate test for gas 

 production than a medium containing 

 bile. 



(b) Ritter used 3.0 g. Liebig's beef 

 extract, 10.0% peptone and 10.0 g. 

 lactose per liter for the presumptive 

 test for B. coli in water analysis. 



(c) Gompertz and Vorkaus used a me- 

 dium containing 4.0 g. Liebig's meat 

 extract, 10.0 g. Merck's peptone and 

 5.0 g. NaCl, and 50.0 g. lactose per 

 liter to cultivate B. acidophilus for 

 therapeutical purposes. The authors 

 reported that an incubation of 60 

 hours gave about one hundred mil- 

 lion bacilli to each cc. of clear cul- 

 ture. The organisms were trans- 

 ferred to a sterile 8 ounce bottle and 

 kept until desired for therapeutic 

 purposes. 



(d) Winslow and Dolloff studied the 

 effect of certain triphenyl-methane 



