CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



279 



dyes on the growth of the colon 

 bacilli. He added 10.0% lactose to a 

 solution of 8.0 g. of Difco dehydrated 

 nutrient broth and added the dyes 

 from a 1.0% stock solution. He re- 

 ported that 

 Rosolic acid in dilution of 1 to 1000 



inhibited. 

 Gentian violet in dilution of 1 to 



5000 to 50,000 inhibited. 

 Brilliant green in dilution of 1 to 

 10,000 to 1,000,000 inhibited. 

 References: Koegel (1914 p. 460), Obst 

 (1916 p. 76), Ritter (1919 p. 610), Gom- 

 pertz and Vorhaus (1921 p. 498), Winslow 

 and Dolloff (1922 p. 303). 



902. Gottheil's Sucrose Extract Broth 



Constituents: 



1. Water 1000.0 cc. 



2. Meat extract (Liebig's) .... 10.0 g. 



3. Peptone 10.0 g. 



4. Sucrose 10.0 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Method not given. 



Use: Cultivation of organisms found in the 

 soil, on roots and on rhizomes. 



Variants: The author gave the following 

 variants: 



(a) Added 0.02% tartaric acid. 



(b) Used 10.0 g. peptone, 10.0 g. Liebig's 

 meat extract and 5.0 g. of sucrose to 

 1200.0 cc. of water and added 5.0 g. 

 glucose, 5.0 g. lactose and 1.0 g. of 

 Seignette salt (Rochelle salt). 



(c) von Wahl used the medium with only 

 1.0 g. sucrose instead of 10.0 g. and 

 variant (b) to cultivate the carrot 

 bacillus (Bacillus daucarum) and 

 other organisms causing vegetable 

 spoilage. 



References: Gottheil (1901 p. 432), von 

 Wahl (1906 p. 495). 



903. Gordon's Starch Extract Broth 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Lemco 10.0 g. 



3. Peptone 10.0 §. 



4. Starch 5.0 g. 



5. Litmus to color 



6. NaHCOa 1.0 g. 



Preparation: 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Tinge with litmus. 

 Sterilization: Not specified. 



Use: Determine fermentation of starch by 

 y. cholerae asiaticae. Author reported 

 that V. cholerae asiaticae produced acid 

 within 24 hours. Vibrio Finkler and 

 Prior gave only a feeble acid reaction 

 after 3 days. Staphylococci, strepto- 

 cocci, B. diphtheriae, B. coli, B. enteri' 

 tides (Gartner), B. typhosus, B. proteus, 

 all failed to ferment starch with the 

 production of acid. 



Reference: Gordon (1906 p. 5). 



904. La Garde's Dextrin Peptone Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone 5.0 g. 



3. Dextrin 5.0 g. 



4. Meat extract trace 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of Phycomyces nitens, 

 Mucor mucedo, Mucor Rouxii, Mucor 

 corymbifer, Mucor spinosus, Mucor 

 rasemosus, Mucor rhizopodiformis, Mucor 

 stolonifer. 



Reference: La Garde (1911-12 p. 248). 



905. Banning's Glycerol Extract Broth 



Constituents : 



1. Water 1000.0 cc. 



2. Glycerin 10.0 g. 



3. Peptone 10.0 g. 



4. Meat extract 10.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Do not adjust the reaction. 

 Sterilization: Method not given. 



Use: To study oxalic acid formation by 

 Bact. Pasteurii, Bact. aceti, Bad. xyli- 

 num, Bact. Dortmund, Bact. ascendens. 

 La Garde used a similar medium for the 

 cultivation of a number of moulds. 



Variant: La Garde used a solution con- 

 taining 10.0 g. peptone, 5.0 g. glycerol 

 and a trace of meat extract per liter for 

 the cultivation of Phycomyces nitens, 

 Mucor mucedo, Mucor Rouxii, Mucor 

 corymbifer, Mucor spinosus, Mucor race- 



