280 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



mosus, Mucor rhizopodiformis, Mucor 

 stolonijer. 

 References : Banning (1902 pp. 395 and 425), 

 La Garde (1911-12 p. 248). 

 906. Jacobson's Mannitol Extract Broth 



Constituents : 



1. Water 1000.0 cc. 



2. Meat extract (Liebig's) .... 10.0 g. 



3. Peptone (Witte's) 10.0 g. 



4. NaCl 5.0 g. 



5. Mannitol 10.0 g. 



6. Litmus solution 50.0 cc. 



Preparation : 



(1) Prepare a sugar free bouillon con- 

 taining 1% Liebig's meat extract, 1% 

 peptone and 0.5% NaCl. 



(2) Add about 20.0 cc. N/1 NaOH to 

 neutralize. 



(3) Boil for about one hour. 



(4) Filter. 



(5) Add 1% mannitol and 5.0% litmus 

 solution. 



(6) After sterilization, distribute in ster- 

 ile tubes. 



Sterilization: Sterilize in a steam bath. 

 Use: To determine fermentation of man- 

 nitol by the typhoid bacilli. 

 Reference: Jacobson (1910 p. 211). 



907. Harrison and van Der Leek's Citrate 

 Aesculin Extract Broth 



Preparation and composition the same 

 as for medium 891 except no bile salt 

 is added. 



908. De Gaetano's Potato Extract Broth 



(Kamen) 



Constituents : 



1. Potato infusion 1000.0 cc. 



2. Meat extract (Liebig's) 20.0 g. 



3. Peptone 10.0 g. 



4. Dextrose 100.0 g. 



5. Tartaric acid 5.0 to 10.0 g. 



Preparation : 



(1) Preparation of potato infusion (Kar- 

 tofi'elaufguss) not given. 



(2) Dissolve 2, 3, 4 in 1. 



(3) Filter. 



(4) Distribute in 6.0 cc. lots in test tubes. 

 Sterilization: Method not given. 



Use : Cultivation of Saccharomyces sep- 



ticus. 

 Reference: De Gaetano (1897 p. 200). 



909. Albus and Holm's Beef Extract 

 Medium (Medium X) 



Constituents : 



1. Water 1000.0 cc. 



2. Yeast (1.0%) 10.0 g. 



3. Peptone (1.0%) 10.0 g. 



4. Lactose (1.0%) 10.0 g. 



5. Beef extract (1.0%) 10.0 g. 



6. Brom cresol purple (5.0% 

 alcoholic soln.) 0.5 cc. 



7. Sodium recinolate. 

 Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Divide in four lots, and keep one as 

 a control. To the other three add 

 varying quantities of sodium recino- 

 late to lower the surface tension. 

 Surface tension determined by weight 

 drop method following sterilization. 



Sterilization: Method not given. 



Use: To determine the eflfect of surface 

 tension on growth of Lactobacillus bul- 

 garicus and Lactobacillus acidophilus. 

 The author reported that L. bulgaricus 

 was inhibited at a surface tension lower 

 than 40 dynes while L. acidophilus ex- 

 hibited good growth in the same media 

 depressed to 36 dynes. 



Variants: The authors used sodium tauro- 

 cholate and sodium glycocholate instead 

 of sodium recinolate to lower the sur- 

 face tension. 



Reference: Albus and Holm (1926 p. 14). 



910. Flint's Serum Extract Broth 



Constituents : 



1. Water 750.0 cc. 



2. Serum, blood 250.0 cc. 



3. Peptone 15.0 g. 



4. Glucose 15.0 g. 



5. Salt 3.0 g. 



6. Meat extract 3.0 g. 



Preparation : 



(1) Mix 1, 2, 3, 4, 5 and 6. 



(2) Make alkaline by the addition of 

 KOH. 



(3) Filter. 



(4) Tube. 



Sterilization: Sterilize in the Arnold. 



Use: Cultivation of streptococci. Author 

 reported that streptococci grew abun- 

 dantly in 24 hours and caused a flaky 



