282 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Use: Isolation of bacteria from infected 



teeth. 

 Reference: Haden (1923 p. 831). 



914. Hoffmann and Fischer's Nutrose 

 Extract Broth (Heinemann) 



Constituents: 



1. Water, tap 1000.0 cc. 



2. Peptone 10.0 g. 



3. Extract, meat 3.3 g. 



4. Nutrose (1.0%) 10.0 g. 



5. Caffein (0.5%) 5.0 g. 



6. Crystal violet 1.0% solution. 

 Preparation : 



(1) Prepare e.xtract broth according to 

 Heinemann's method using 1, 2 and 

 3 (see medium 695). 



(2) Dissolve 1.0% nutrose, 0.5% caffein 

 and 1.0% solution of crystal violet 

 in (1). 



Sterilization: Not specified. 



Use: Enrichment of colon typhoid group. 



Reference: Heinemann (1905 p. 130). 



915. Keim's Honey Extract Broth 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone 10.0 g. 



3. Meat extract 10.0 g. 



4. Honey 25.0 g. 



5. NaCl 5.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Neutralize. 

 Sterilization: Method not given. 

 Use: Substitute for glucose broth. 

 Reference: Keim (1923 p. 604). 



916. Otaki and Akimoto's Sodium Citrate 

 Blood Extract Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Meat extract 10.0 g. 



3. Peptone 10.0 g. 



4. Saponin (Merck) 1.0 g. 



5. Sodium citrate (neutral) . . . 20.0 g. 



6. Blood. 

 Preparation : 



(1) Dissolve 2 and 3 in 1 by boiling. 



(2) Neutralize (1). 



(3) Add 4 and 5 to (2). 



(4) To 5.0 cc. of (3) add 2.0 cc. of blood. 

 Sterilization: Not specified in the abstract. 



Use: Enrichment of typhoid from the 

 blood. Saponin is added to lake the 

 erythrocytes. 



Reference : Otaki and Akimoto (1922 p. 101) 

 taken from (1923 p. 331). 



917. Dimitroff's Egg Sea Water Medium 



Constituents : 



1. Sea water (75.0%) 750.0 cc. 



2. Extract broth (25.0%) 250.0 cc. 



3. Egg white. 

 Preparation: 



(1) Place small cubes of hard boiled egg 

 white in 10.0 cc. of beef extract broth. 



(2) Mix 25.0% of sterile (1) with 75.0% 

 sterile sea water. 



Sterilization: Method not given. 



Use: Cultivation of Spirillum virginianum. 



This medium supported life of the 



organism for a few days. 

 Variants : 



(a) The following medium gave good 

 results, according to the author. 



Physiological salt solution. .. 200.0 cc. 

 Egg cube medium (same as 



(1)) 50.0 CO. 



Liquid 2.0% agar in distilled 



water 30.0 cc. 



(b) The author reported that the follow- 

 ing semisolid medium gave a luxu- 

 riant growth of the organism. The 

 spirilla grew luxuriantly in this me- 

 dium even when the egg cube medium 

 was omitted. 



Liquid 2.0% agar in distilled 



water 40.0 cc. 



Distilled water 68.0 cc. 



Egg cube medium (same as (1)) 20.0 cc. 

 Reference: Dimitroff (1926 p. 21-22). 



918. Lohnis' Urea Bouillon 



Constituents : 



1. Bouillon 1000.0 cc. 



2. Urea (2.0 or 10.0%) 20.0 or 100.0 g. 

 Preparation: (1) Dissolve 2.0% or 10.0% 



urea in bouillon. 

 Sterilization: Sterilize in the usual manner. 

 Use: Demonstrate the decomposition of 



urea. 

 Variants : 



(a) Geilinger sterilized the bouillon, 

 added a sterile watery urea solution 

 to the bouillon to obtain the 10.0% 

 strength of urea. The urea solution 



