CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



283 



was sterilized by heating at one-half 



atmosphere pressure for 30 minutes. 



(b) Cunningham added 2.0, 5.0 or 10.0% 



urea to bouillon and sterilized by the 



intermittent method in flowing steam. 



Reference: Lohnis (1913 p. 95), Geilinger 



(1917 p. 246), Cunningham (1924 p. 143). 



919. Baruchello's Caffein Bouillon 



Constituents: 



1. Water 650 .0 cc. 



2. Bouillon 350.0 cc. 



3. Caffein 10-0 g- 



Preparation : 



(1) Method of preparation of bouillon 

 not given, 



(2) Mix 1, 2 and 3. 



(3) Adjustment of reaction not specified. 

 Sterilization: Sterilize in the usual manner. 

 Use: Caffein hindered development of 



B. coll. 

 Reference: Baruchello (1905 p. 570). 



920. Picker's Crystal Violet Caffein 



Bouillon (Bezanfon) 



Constituents : 



1. Meat infusion 1000.0 cc. 



2. Peptone 30.0 g. 



3. NaCl 2.5 g. 



4. Caffein 6.0 g. 



5. Crystal violet 0.0007 g. 



Preparation: 



(1) Dissolve 2, 3, 4 and 5 in meat infusion. 



(2) Neutralize to phenolphthalein by 

 adding 21.0 cc. of 10 normal soda 

 solution. 



Sterilization: Not specified. 



Use: Enrichment of the colon-typhoid 



group. 

 Reference: Bezangon (1920 p. 345). 



921. Miiller's Lactose Ferro-cyanide 

 Bouillon 



Constituents: 



1. Bouillon 1000.0 cc. 



2. Lactose 25.0 g. 



3. Potassium ferro-cyanide ... . 30.0 cc. 



4. Extrait de fer pomme 30.0 cc. 



Preparation : 



(1) Prepare bouillon. 



(2) Neutralize (2) and add 10.0 cc. of 

 normal NaOH per liter. 



(3) Add 25.0 g. sterile lactose to ster- 

 ile (2). 



(4) Prepare a solution of 1 part "Extrait 

 de fer pomme" to 100. 



(5) Prepare a solution of 1 part potas- 

 sium ferro-cyanide to 100. 



(6) Mix 1000.0 cc. (3), 30.0 cc. of sterile 

 (4) and 30.0 cc. of sterile (5) under 

 aseptic conditions. 



(7) Distribute as desired into sterile 

 containers. 



Sterilization: Method of sterilization of 

 (2), lactose, (4) or (5) not given. 



Use: Differentiation of colon-typhoid ba- 

 cilli. Author reported that colon bacilli 

 caused a blue precipitate to develop. 

 Typhoid bacilli caused no change in 

 color, a brown precipitate formed. 



Reference: Muller (1922 p. 984). 



922. Frost's Glucose Bouillon 



Constituents : 



1. Bouillon 1000.0 cc. 



2. Glucose (1.0%) 10.0 g. 



Preparation : 



(1) Prepare bouillon and add 1.0% 

 glucose. 



(2) Tube in test tubes or fermentation 

 tubes. 



Sterilization: Sterilize in the steamer. 

 Use: General culture medium. 

 Variants : 



(a) Kendall, Day and Walker specified 

 the use of a sugar free bouillon. 



(b) Stitt added small pieces of CaCOa 

 (marble) to tubes of glucose bouillon. 



(c) Klimmer used a 0.5% glucose bouil- 

 lon, tubed in 5.0 cc. lots. After 

 inoculation and incubation, 3 drops 

 of the following indicator solution 

 was added to each tube: 



1. Distilled water 22.0 cc. 



2. 2.0% watery indigo 2.0 cc. 



3. Carmine solution 



4. 10.0% acid fuchsin solution. . 1.0 cc. 



Klimmer differentiated pseudo diph- 

 theria and diphtheria bacilli in this 

 medium. He reported uninoculated 

 bouillon blue, diphtheria culture red 

 and pseudo diphtheria culture green 

 after a few minutes. Pseudo diphthe- 

 ria culture red also after 36 hours. 

 References: Frost (1903 p. 64), Heinemann 

 (1905 p. 127), Gage (1908 p. 282), Bornand 

 (1913 p. 516), Kendall, Day and Walker 

 (1914 p. 419), Stitt (1921 p. 35), Klimmer 

 (1923 p. 223). 



