CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



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2. Glucose 20.0 g. 



3. Acetic acid 5.0 g. 



Preparation: (1) Add 2 parts glucose, and 



0.5 parts acetic acid to bouillon. 



Sterilization: Not specified. 



Use: Enrichment of aciduric bacilli from 

 stools. Heinemann and Hefferan iso- 

 lated B. vulgaricus . 



Variants: Heinemann and Hefferan spec- 

 ified the use of glacial acetic acid. 



References: Finkelstein (1900 p. 263), 

 Heinemann and Hefferan (1909 p. 308). 



927. Savage's Neutral Red Glucose Bouillon 



Constituents: 



1. Bouillon 1000.0 cc. 



2. Glucose (0.5%) 5.0 g. 



3. Neutral red (Grubler's 0.5% 



soln.) 10.0 cc. 



Preparation : 



(1) Add 0.5% glucose to bouillon. 



(2) Prepare a 0.5% watery solution of 

 Grubler's neutral red. 



(3) Add 0.1 cc. of (3) to every 10.0 cc. 

 of (1). 



Sterilization: Method not given. 



"Use: Detection of Bacillus coli in water 

 analysis. (Neutral red reaction.) Ba- 

 cillus coli gave a yellow coloration to the 

 medium. 



References: Savage (1901 p. 437), Gage and 

 Phelps (1902 p. 407), Irons (1902 p. 315). 



928. DeKorti's Oleic Acid Glucose Bouillon 



Constituents : 



1. Bouillon 1000.0 cc. 



2. Oleic acid (1.0%) 10.0 g. 



3. Glucose (1.0%) 10.0 g. 



Preparation : 



(1) Prepare ordinary bouillon. 



(2) Add 1.0% oleic acid and 1.0% glu- 

 cose to (1). 



(3) Tube in 10.0 cc. quantities. 



(4) Pour a layer of vaseline on the sur- 

 face of the broth in each tube. 



Sterilization: Method not given. 



Use: Enrichment of acne bacilli. Medium 

 inoculated by adding a suspension of a 

 comedo crushed in a little normal salt 

 solution. Seal the hole made by the 

 pipette by means of heat. Author re- 

 ported that after incubation at 37° for 

 4 days, large numbers of acne bacilli were 

 found present. 



Reference: De Korti (1921 p. 250). 



929, Plimmer's Tartaric Acid Infusion Broth 



Constituents : 



1. Infusion broth 1000.0 cc, 



2. Glucose 20.0 g. 



3. Tartaric acid 10.0 g. 



Preparation : 



(1) Prepare infusion broth in the usual 

 manner. 



(2) Neutralize carefully. 



(3) Dissolve 2 and 3 in (2). 

 Sterilization: Method not given. 

 Use: Enrichment of Krebs-organisms. 

 Reference: Plimmer (1899 p. 806). 



930. Gage's Lactose Bouillon 



Constituents : 



1. Bouillon 1000.0 cc. 



2. Lactose 10.0 g. 



3. Neutral red 0.5% soln 20.0 cc. 



Preparation: 



(1) Exact method of preparation of bouil- 

 lon not given. 



(2) Adjust (1) to +0.8. 



(3) Add 1.0% lactose and 2.0% neutral 

 red (0.5% aqueous solution). 



(4) Filter. 



(5) Tube. 



Sterilization: Method not given. 



Use: Primarily for the presumptive test for 

 Bacillus coli in water analysis. Author 

 reported that Bacillus coli produced acid 

 and a yellowish green fluorescence. 



Variants : 



(a) Koegel omitted the neutral red and 

 used 6.0% lactose instead of 1.0%. 

 The medium was used for the culti- 

 vation of bulgaricus. 



(b) Cunningham used 1.0% of Andrades 

 indicator and 0.5% lactose instead of 

 neutral red and 1.0% lactose. 



References: Gage (1908 p. 282), Koegel 

 (1914 p. 460), Cunningham (1924 p. 102). 



931. Hall and Ellef son's Gentian Violet 

 Lactose Broth 

 Constituents: 



1. Water 1000.0 cc. 



2. Bouillon 1000.0 cc. 



3. Lactose (1.0%) 10.0 g. 



4. Gentian violet, 1:100,000 or 1:20,000 

 Preparation : 



(1) Prepare 2.0% lactose bouillon. 



(2) Add an equal volume of water to (1) 

 giving a 1.0% concentration of 

 lactose. 



