286 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(3) Add 1 to 100,000 or 1 to 20,000 of 

 gentian violet. 



Sterilization: Not specified. 



Use: Presumptive test for B. coli. Author 

 reported that dye inhibited other organ- 

 isms. 



References : Hall and Ellefson (1918 p. 337), 

 Levine (1921 p. 110). 



932. Jouan's Citrate Lactose Bouillon 

 (Besson) 



Constituents: 



1. Water 850.0 cc. 



2. Bouillon 150.0 cc. 



3. Lactose 10.0 g. 



4. Magnesium citrate 1-0 g. 



5. Litmus. 

 Preparation : 



(1) Dissolve 150.0 cc. of bouillon, 10.0 g. 

 lactose and 1.0 g. of magnesium ci- 

 trate in 850.0 cc. of water. 



(2) Add sufficient litmus solution to give 

 a violet color. 



(3) Filter. 



(4) Tube. 



Sterilization: Sterilize at 110 to 112°C. 

 Use: Synthetic whey. 

 Reference: Besson (1920 p. 208). 



933. Wollman's Starch Bouillon 



Constituents: 



1. Water 600.0 cc. 



2. Bouillon 400.0 cc, 



3. Starch 30.0 g. 



4. Peptone (Chapoteaut) 2.0 g. 



Preparation : 



(1) Prepare bouillon. 



(2) Add 400.0 cc. of (1) to 600.0 cc. of 

 water. 



(3) Dissolve 3 and 4 in (2). 

 Sterilization: Not specified. 



Use: Cultivation of Glycobacter proteo- 

 lytic u:^. 

 Reference: Wollman (1912 p. 616). 



934. Holzel's Glycogen Bouillon 



Constituents : 



1. Bouillon 1000.0 to 1500.0 cc. 



2. Glycogen 20.0 to 100.0 cc. 



Preparation : 



(1) Prepare sugar free bouillon. 



(2) Add 10.0 to 15.0 cc. of (1) to 0.2 to 

 1.0 g. of Merck's glycogen. 



(3) Cool quickly after sterilization. 



Sterilization: Sterilize in the autoclave in 



the usual manner. 

 Use : Cultivation of anthrax bacilli. 

 Reference: Holzel (1913 p. 149). 



935. Heinemann's Glycerol Bouillon 



Constituents : 



1. Bouillon 1000.0 cc. 



2. Glycerol (6.0%) 60.0 g. 



Preparation: (1) Add 6.0% glycerol to 



bouillon. 

 Sterilization: Not specified. 

 Use: General culture medium. 

 Variants : 



(a) Kendall, Day and Walker specified 

 the use of sugar free bouillon and 

 used 30.0 g. of glycerol instead of 

 60.0 g. per liter. 



(b) Owen also used a 3.0% glycerol bouil- 

 lon to determine the amount of 

 alcohol formed in the fermentation 

 of glycerol by Bacillus saccharalis. 



References: Heinemann (1905 p. 127), 

 Kendall, Day and Walker (1914 p. 419), 

 Owen (1916 p. 241), Dopter and Sac- 

 quepee (1921 p. 119), Stitt (1923 p. 35), 

 Cunningham (1924 p. 165). 



936. Kendall, Day and Walker's Mannitol 

 Bouillon 



Constituents : 



1. Bouillon 1000.0 cc. 



2. Mannitol 10.0 g. 



Preparation : 



(1) Prepare nutrient sugar free bouillon, 



(2) Dissolve 2 in 1. 



(3) Distribute in 100.0 cc. lots. 

 Sterilization: Not specified. 



Use: To study the metabolism of tubercle 

 bacilli. Authors reported that they used 

 alizarin, neutral red and phenolphthalein 

 to study changes in reaction; Ziehl- 

 Neelsen stain for staining. 



Reference : Kendall, Day and Walker (1914 

 p. 419). 



937. Giltner's Adonitol Bouillon 



Constituents : 



1. Bouillon 1000.0 cc. 



2. Adonitol (0.5%) 5.0 g. 



Preparation: (1) Dissolve 0.5% adonitol in 



bouillon. 

 Sterilization: Not specified. 



