CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



287 



Use: Differentiation of colon-aerogenes 

 group. Author reported that B. aero- 

 genes and B. cloacae fermented adonitol. 



Reference: Giltner (1921 p. 383). 



938. Vincent's Phenol Bouillon 



Constituents : 



1. Bouillon. 



2. Phenol. 

 Preparation : 



(1) Preparation of bouillon not given. 



(2) Distribute in 10.0 cc. lots in tubes. 



(3) To each tube add 5 drops of a 5.0% 

 phenol solution (1 drop of 5.0% phenol 

 to each 2.0 cc. bouillon). 



Sterilization: Not specified. 



Use: Enrichment of typhoid bacilli. 

 Water analysis. Add 5 to 10 drops of the 

 water under investigation to each tube. 

 Author reported that contaminated water 

 clouded the medium. Other organisms 

 were inhibited. To obtain a pure cul- 

 ture of typhoid bacilli 3 or 4 sub-cultures 

 were made in this medium. 



Variants: Kamer added 5.0 g. of phenol 

 and 4.0 g. of pure HCl to 100.0 cc. dis- 

 tilled water and then added 4, 6 or 9 drops 

 (30 drops = 60 cc.) of this mixture to 

 10.0 cc. of sterile bouillon contained in 

 tubes. 



References: Vincent (1890 p. 63), (1900 

 p. 45), Kamer (1892 p. 35). 



939. Thjotta and Avery's Yeast Bouillon 



Constituents : 



1. Distilled water 400.0 cc. 



2. Brewer's yeast 100.0 g. 



3. Plain broth 

 Preparation : 



(1) Exact method of preparation or com- 

 position of plain broth not given. 



(2) Adjust the reaction of (1) to pH = 7.8. 



(3) Emulsify 100.0 g. of brewers yeast in 

 400.0 cc. of distilled water with the 

 reaction of pH = 4.6. 



(4) Boil over a free flame for 10 minutes. 



(5) Allow to sediment at room tem- 

 perature. 



(6) Pipette off the clear supernatant 

 extract and test sterility. 



(7) Store in ice chest until ready for use. 

 Readjust the reaction of the extract 

 from pH = 7.3 to 7.5 just before use. 



Sterilization: Sterilization of yeast extract 

 effected in step (4) in the preparation. 

 Sterilization of bouillon not specified. 



Use : To study bacterial nutrition of Bacil- 

 lus influenzae. Authors reported that the 

 medium supported the growth of B. 

 influenzae. Growth accessory materials 

 resisted boiling for 10 minutes, were 

 destroyed by autoclaving, would pass 

 thru a Berkefeld filter but were absorbed 

 by charcoal. Broth containing extract 

 alone, however, did not give continued 

 growth of the influenza bacilli. Primary 

 inoculations were made from blood broth. 



Reference: Thjotta and Avery (1921 

 p. 100). 



940. Thjotta and Avery's Tomato Bouillon 



Constituents: 



1. Bouillon. 



2. Tomatoes. 

 Preparation: 



(1) Preparation of bouillon not given. 



(2) Readjust the reaction to pH = 7.8. 



(3) Sear the skin off a ripe tomato with 

 a red hot knife. 



(4) Plunge a sterile fork thru this area. 



(5) Dip the tomato into alcohol and 

 flame off, then plunge into boiling 

 water. 



(6) Remove the peeling with sterile 

 forceps. 



(7) Place the tomatoes in a sterile 

 enamel dish and crush with a sterile 

 pestle. (Reaction of juice pH = 

 4.2.) 



(8) Boil the juice 10 minutes. 



(9) Filter the juice thru a Berkefeld 

 candle, or clear by centrifugation. 



(10) Test sterility and store in its original 

 acidity. 



(11) Just before use readjust the reaction 

 from pH = 7.3 to 7.5. 



(12) Make dilutions of the extract in (2) 

 in the proportion of 1:10 and 1:100. 



Sterilization: As indicated in steps (8) 

 and (9) of the preparation. Sterilization 

 of the bouillon not specified. 



Use: To study bacterial nutrition of 

 Bacillus influenzae. Authors reported 

 that the medium supported growth of 

 B. influenzae. Growth accessory ma- 

 terials resisted boiling for 10 minutes, 

 were destroyed by autoclaving, would 



