290 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Sterilization: Sterilization of bouillon not 



given. 

 Use : To study bacterial nutrition of Bacillus 



influenzae. Author reported no growth 



in dilution of 1 to 10,000 of blood cells. 

 Reference: Thjotta and Averj' (1921 



p. 109). 



947. Smith, Brown and Walker's Tissue 

 Bouillon 



Constituents : 



1. Bouillon. 



2. Tissue, Rabbit or Guinea Pig. 

 Preparation : 



(1) Prepare ordinary bouillon. 



(2) Tube in fermentation tubes. 



(3) Kill a rabbit or guinea pig by chloro- 

 form. 



(4) Moisten the animal thoroly with 

 water. 



(5) Reflect the skin and open the abdo- 

 men with sterile instruments. 



(6) Remove the liver, spleen, kidneys and 

 bits of tissue with sterile forceps. 



(7) Place these bits of tissue, just large 

 enough to pass into the narrow part 

 of the fermentation tubes. The tis- 

 sue is pushed into this narrow por- 

 tion, but not necessarily beyond. 



(8) Incubate two or more days to test 

 sterility. 



Sterilization: Sterilization of bouillon not 

 given. 



Use: Cultivation of anaerobes and flag- 

 ellates. 



Variants : 



(a) Wrzosek reported that the addition of 

 sterile tissue to a medium permitted 

 the growth of obligate anaerobes. 



(b) Harrass sterilized at 100 °C. for 90 

 to 120 minutes the medium after the 

 addition of animal tissue. He also 

 suggested the use of water instead of 

 bouillon. 



(c) Grozony diluted equal parts of bouil- 

 lon (containing 2.9% peptone) and 

 water, and made slightly alkaline by 

 the addition of sodium carbonate. 

 After sterilization in the autoclave, 

 cooled to 45°C. and a small piece of 

 sterile dog kidney was added to each 

 tube under aseptic conditions. The 

 medium was used for the cultivation 

 of flagellates. 



(d) Bull and Pritchett added fragments 



of sterile skeletal muscle of the pigeon 



or rabbit to 100.0 cc. quantities of 



bouillon. They used the medium for 



the production of toxin by Bacillus 



welchii. Medium incubated in a 



vacuum jar from which the oxygen 



has been removed. They also used 



the above medium with the addition 



of 0.0 to 1.0% glucose, 0.2 or 0.3% 



giving the best results. 



References: Smith, Brown and Walker 



(1905-06 p. 196), Wrzosek (1905 pp. 1268- 



1270), Harrass (1906 p. 2237), Bull and 



Pritchett (1917 pp. 129, 871, 873, 875), 



G6zony (1920 p. 566). 



948. Orr's Glucose Heart Bouillon 



Constituents: 



1. Bouillon 1000.0 cc. 



2. Glucose (1.0%) 10.0 g. 



3. Heart, beef 100.0 g. 



4. Marble 

 Preparation : 



(1) Prepare 1.0% glucose bouillon. 



(2) Add (1) to 100.0 g. of finely minced 

 beef heart. 



(3) Add a quantity of marble chips. 



(4) Adjust to pH = 8.0. 



(5) Cover with a layer of paraffin oil. 

 Sterilization: Method not given. 



Use: Cultivation of B. botulinus and toxin 

 production. Author reported that 

 M.L.D. varied from 0.0001 cc. to 0.05 cc. 

 depending on the strain used. 



Reference: Orr (1920-21 p. 128). 



949. Park, Williams and Krumwiede's Meat 

 Medium 



Constituents : 



1. Bouillon. 



2. Meat. 



Preparation: (1) Drop pieces of meat into 



tubes of bouillon. 

 Sterilization: Method not given. 

 Use: General culture medium. 

 Reference : Park, Williams and Krumwiede 



(1924 p. 125). 



950. Kligler's Tissue Infusion Bouillon 



Constituents : 



1. Bouillon. 



2. Physiological salt solution. 



3. Tissue. 



