CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



291 



Preparation: 



(1) Prepare bouillon. 



(2) Obtain tissue (beef heart, rabbit or 

 cat tissue or organ or mucosa of 

 various organs were used) as free 

 from blood as possible under aseptic 

 conditions. 



(3) Wash (2) with saline solution to re- 

 move all visible traces of blood. 



(4) Weigh and chop into small bits and 

 suspend in 9 times the weight of 

 saline solution. 



(5) Shake thoroly and place in the ice 

 box over night. 



(6) Centrifuge and filter thru a Berkefeld 

 candle. 



(7) Tube (1) in 5.0 cc. lots. 



(8) Add from 0.01 cc. to 1.0 cc. of sterile 

 (6) to each tube of sterile (7). 



Sterilization: Sterilize (6) by filtering thru 



a Berkefeld filter. Sterilization of (7) not 



specified. 

 Use: To show influence of growth accessory 



substances on growth of pathogenic 



bacteria. 

 Reference: Kligler (1919 p. 32). 



951. Capaldi's Egg Yolk Bouillon 



Constituents : 



1. Bouillon. 



2. Egg yolk. 

 Preparation : 



(1) Break an egg. Separate the white 

 from the yolk and place the yolk in a 

 sterile Petri dish. 



(2) Exact method of preparation of bouil- 

 lon not given. 



(3) Place a glowing glass rod on a spot on 

 the covering of the egg yolk. (This 

 sterilizes the covering of the naturally 

 sterile yolk.) 



(4) Remove 3-4 loops ("oesen"), (exact 

 amount not given) of egg yolk thru 

 the portion of covering of the yolk 

 that had been sterilized and add to 

 each tube of (3). 



Sterilization: Sterilization of bouillon not 

 given. 



Use: Cultivation of tubercle bacilli and 

 diphtheria bacilli to study toxin pro- 

 duction by diphtheria bacilli. 



Reference: Capaldi (1896 p. 801). 



952. Oberstadt's Egg Albumin Bouillon 



Constituents : 



1. Bouillon. 



2. Egg albumin. 

 Preparation : 



(1) Boil eggs 10 minutes. 



(2) Shell the egg, separate the yolk and 

 cut the white into small pieces. 



(3) To 100.0 cc. of clear ordinary nutrient 

 bouillon that is slightly alkaline to 

 litmus add the finely chopped albumin 

 from one egg. 



(4) Heat at 100°C. for 30 minutes and 

 allow to stand until the following day. 



(5) Filter off the egg albumin. 



(6) Distribute the filtrate as desired. 

 Sterilization: Autoclave at 0.5 atmospheres 



pressure for 45 minutes. If the medium 

 is not to be used within 8 days, sterilize 

 the medium at 0.5 atmospheres pressure 

 without filtering the egg albumin. Then 

 filter just before ready to use. 



Use: General culture medium. Author re- 

 ported that growth of pathogenic and 

 saphrophytic organisms was much better 

 on this medium than on ordinary bouillon. 



References: Oberstadt (1914-15 p. 137), 

 Klimmer (1923 p. 202). 



953. Lipschutz's Egg Albumin Bouillon 



Constituents : 



1. Water 100.0 cc. 



2. Egg albumin 2.0 g. 



3. Bouillon 200.0 to 300.0 cc. 



Preparation : 



(1) Prepare a 2.0% egg albumin solution 

 in water. 



(2) Add 20.0 cc. of 0.1 normal lye solution 

 for each 100.0 cc. of (1). 



(3) Allow to stand for 30 minutes shaking 

 occasionally. 



(4) Filter and distribute into Erlen- 

 meyer flasks in 30 to 50.0 cc. lots. 



(5) Mix 1 part sterile (4) with 2 or 3 

 parts sterile bouillon. 



Sterilization: Sterilize (4) by placing on an 

 asbestos plate and bringing to a boil. 

 This may be done on the same or suc- 

 cessive days, or the solution may be 

 sterilized in the steamer. Method of 

 sterilization of bouillon not given. 



Use: Cultivation of gonococci. 



Reference: Lipschlitz (1904 p. 744). 



