CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



297 



Preparation : 



(1) Dissolve Merck's mucin in bouillon. 

 (Water or nutrient agar may be used 

 instead of bouillon.) 



(2) Concentrate the solution by slow 

 evaporation to obtain the desired 

 strength of mucin. 



Sterilization: Not specified. 

 Use: Cultivation of parasitic and sapro- 

 phytic bacteria. 

 Reference: Mayer (1899 p. 818). 



970. Schloffer's Urine Bouillon 



Constituents : 



1. Bouillon 2000.0 cc. 



2. Urine 1000.0 cc. 



Preparation : 



(1) Prepare bouillon. 



(2) Mix 2 parts sterile bouillon and 1 part 

 sterile urine. 



(3) Tube or flask. 



Sterilization: Obtain urine under sterile 

 conditions. The urine may be collected 

 in sterile flasks and then heated to 70° 

 to 80°C. to sterilize. 



Use: Cultivation of diphtheria bacilli. 

 Authors reported that growth a little 

 more luxuriant than on ordinary bouillon. 



Reference: Schloffer (1893 p. 659). 



971. Kligler's Nasal Secretion Bouillon 



Constituents : 



1. Bouillon. 



2. Nasal secretions. 

 Preparation : 



(1) Prepare bouillon. 



(2) Obtain nasal washings by using 50.0 

 cc. warm saline solution for each 

 washing of apparently normal in- 

 dividuals. 



(3) Collect the washings in sterile bottles. 



(4) Test sterility and tube in 2.0 cc. lots 

 in small test tubes. 



(5) Mix 0.1 cc. of (4) with 5.0 cc. of (1). 

 Sterilization: Method of sterilization of 



bouillon not given. Sterilize (3) by 

 filtering thru a Berkefeld filter. 



Use: To show influence of nasal washing on 

 growth of pathogenic bacteria. Author 

 reported that nasal secretions of some in- 

 dividuals stimulated the growth of some 

 organisms. Saliva treated in a similar 

 manner did not. 



Reference: Kligler (1919 p. 40). 



972. Thjotta and Avery's Yeast Ascitic Fluid 

 Bouillon 



Constituents : 



1. Distilled water 400.0 cc. 



2. Brewer's yeast 100.0 g. 



3. Bouillon 



4. Ascitic fluid 

 Preparation : 



(1) Exact composition of bouillon not 

 given. 



(2) Adjust the reaction to pH = 7.8. 



(3) Emulsify 100.0 g. of brewer's yeast 

 in 400.0 cc. distilled water with the 

 reaction of pH = 4.6. 



(4) Boil over a free flame for 10 minutes. 



(5) Allow to sediment at room tempera- 

 ture. 



(6) Pipette off the clear supernatant 

 extract and test sterility. 



(7) Store in ice chest until ready for use. 

 Readjust the reaction of the extract 

 from pH = 7.3 to 7.5 just before use. 



(8) Dilute hemoglobin free ascitic fluid 

 with (2) in varying proportions from 

 1:2 to 1:100. 



(9) Add varying amounts of the yeast 

 extract (amount not given) to (8). 



Sterilization: Sterilization of bouillon not 

 given. Yeast infusion is sterilized in the 

 process of preparation in step (4). 



Use: To study bacterial nutrition of Bacil- 

 lus influenzae. Author reported no 

 growth of B. influenzae without yeast 

 extract. Growth in dilutions of ascitic 

 fluid in 1:2 and 1:5 only. 



Reference : Thjotta and Avery (1921 p. 109) . 



973. Thjotta and Avery's Yeast Serum 

 Bouillon 



Constituents : 



1. Distilled water 400.0 cc. 



2. Brewer's yeast 100.0 g. 



3. Bouillon 



4. Serum 

 Preparation : 



(1) Exact method of preparation of 

 bouillon not given. 



(2) Adjust the reaction of (1) to pH 

 = 7.8. 



(3) Emulsify 100.0 g. of brewer's yeast 

 in 400.0 cc. distilled water with the 

 reaction of pH = 4.6. 



(4) Boil over a free flame for 10 minutes. 



