CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



301 



Douglas Trypsin Broth (Hartley).. 1123 

 Ds. Casein or milk digest specified. 



Harvey's Basal Trypsinized Casein 

 Solution 1124 



Teruuchi and Hida's Trypsinized 

 Casein Solution 1125 



Zipfel's Trypsinized Casein Solu- 

 tion 1126 



Bacto Tryptophane Broth (Dehy- 

 drated) 1127 



Bacto Peptonized Milk (Dehy- 

 drated) 1128 



Mueller's Trypsinized Casein Solu- 

 tion 1129 



Cole and Onslow's Trypsinized Ca- 

 sein Solution 1130 



Berman and Rettger's Trypsinized 

 Casein Solution 1131 



Norris' Trypsinized Caseinogen 

 Solution 1132 



Cannon's Trypsinized Casein Solu- 

 tion (Norton and Sawyer) 1133 



De. Digest of a variety of materials spec- 

 ified. 



Duval and Harris' Tryptic Digest 

 Solution 1134 



Harvey's Tryptic Digest Solution. . 1135 



Stickel and Meyer's Tryptic Digest 



Solution 1136 



C3. Both peptic and tryptic digests added. 



Stickel and Meyer's Digest Solution 1137 



Frieber's Digest Extract Solution. . 1138 

 A3. Containing digests of both plant and 



animal origin. 



Davis and Ferry's Hydrolyzed Gli- 

 adin Solution 1139 



Robinson and Rettger's Hydrolyzed 

 Edestin Casein Solution 1140 



978. Kammen's Yeast Digest (Klimmer) 



Constituents : 



1. Water 20,000.0 cc. 



2. Yeast 10,000.0 g. 



Preparation : 



(1) Autoclave 10,000.0 g. yeast with 20 

 liters of water for 2 hours at 1.5 to 1 

 atmospheres pressure. 



(2) Allow to settle and decant the liquid. 



(3) Add pepsin and about 10 liters of 

 0.5% HCl solution to the thick yeast 

 residue from (2). 



(4) Incubate (3) for 5 days at 37°C. 



(5) Allow to settle and decant the liquid 

 or filter. 



(6) Neutralize the liquid by the addition 

 of NaOH. 



(7) Heat at 50° until all the liquid has 

 evaporated. 



(8) Heat at 100 to 105°C. for one hour. 



(9) Pound to a powder. 

 Sterilization: Material remains sterile in 



powdered form. 



Use: Substitute for meat peptone. 



Variants : 



La) Klimmer added the supernatant fluid 

 of (2) to the liquid obtained in step 

 (6) and prepared a powder as indi- 

 cated above, 

 (b) Klimmer dissolved 10.0 g. of the 

 powder obtained above, 8.0 g. NaCl 

 and 0.1 g. KNO3 in a liter of water. 

 He specified that this medium could 

 be used as ordinary bouillon or might 

 be used in the preparation of special 

 media. 



Reference: Klimmer (1923 p. 170). 



979. Ayers and Rupp's Yeast Digest 



Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Yeast 100.0 g. 



Preparation : 



(1) Add 100.0 g. dry fresh yeast to 

 1000.0 cc. distilled water with 1.0 g. 

 pepsin. Add HCl to obtain pH = 

 4.4. 



(2) Incubate at 40° for 24 hours. 



(3) Steam for 30 minutes. 



(4) Filter. 



(5) Dilute to 1000.0 cc. with distilled 

 water. 



(6) Adjust reaction to pH = 7.5. 

 Sterilization: Method not given. 



Use: General culture medium. Author re- 

 ported that this medium gave very good 

 growth of the delicately growing strepto- 

 cocci. 



Reference: Ayers and Rupp (1920 p. 96). 



980. Robertson's Glucose Asparagin 



Solution 



Constituents: 



1. Distilled water (sterile). . . . 1000.0 cc. 



2. Asparagin (Merck) 3.4 g. 



3. CaCl2 0.1 g. 



4. Glucose 20.0 g. 



5. MgS04 0.2 g. 



