CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



311 



(13) Filter and neutralize with ammonia. 



(14) Distribute in flasks and sterilize. 

 Sterilization: Method not given. 



Use: General culture medium and substi- 

 tute for commercial peptone. 



Variants: The author prepared a dry ma- 

 terial by treating the medium obtained 

 above as follows: 



(1) Neutralize the digest with Ba(0H)2 

 (about 40.0 g.) and boil until the 

 upper fluid is clear. 



(2) The reaction should be slightly acid, 

 showing a light excess of H2SO4. 



(3) Allow the digest to settle. 



(4) Pour off the clear solution and filter 

 the remainder, washing the pre- 

 cipitate with water. 



(5) This gives a light yellow solution con- 

 taining about 10.0% peptone. 



(6) This fluid may be evaporated over a 

 water bath or in a vacuum to a thick 

 syrupy paste and then drawn into 

 threads and dried in a desiccator. 



(7) This peptone is equal to Witte's pep- 

 tone in the preparation of media. 



Reference: Bramigk (1921 p. 429). 



1005. Harvey's Blood Clot Digest 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Blood. 



3. Pig's stomach 100.0 g. 



4. HCl 10.0 g. 



Preparation : 



(1) Procure ox or sheep blood from the 

 slaughter house. 



(2) Allow to clot. 



(3) Decant and store the serum in an 

 ice chest. 



(4) Mince the clot. 



(5) Add 100.0 g. clot to 1000.0 cc. tap 

 water. 



(6) Raise slowly to boiling temperature. 



(7) Boil 10 minutes. 



(8) Cool to 50°C. 



(9) Add 100.0 g. minced pig's stomach 

 and 10.0 cc. strong hydrochloric acid. 



(10) Digest 20 hours. 



(11) Make a biuret and a tryptophane 

 test. 



(12) Boil 10 minutes to stop the digestion. 



(13) Pour the mixture on to a wet, thick, 

 clean cloth. 



(14) Collect the fluid which drains thru 

 the cloth, together with that ob- 

 tained by squeezing the cloth. 



(15) Filter the fluid collected thru well- 

 wetted, thick, filter paper. 



(16) Bring the volume up to 1000.0 cc. 



(17) Filter thru well wetted, thick filter 

 paper. 



(18) Make the reaction neutral to litmus. 



(19) Add 75.0 cc. of the decanted serum 

 (3). _ 



(20) Steam sixty minutes. 



(21) Allow the clot to form a compact 



(22) Decant the clear fluid. 

 Sterilization: Sterilize at 100°C. 

 Use: General culture medium. 

 Reference: Harvey (1921-22 p. 100). 



1006. Martin's Stomach Digest Infusion 

 Broth 



Constituents: 



1. Water 2000.0 cc. 



2. Veal 500.0 g. 



3. NaCl 5.0 g. 



4. Stomach (hog) 200.0 g. 



Preparation : 



(1) Infuse 500.0 g. of finely ground veal 

 with 1000.0 g. water for 20 hours 

 at35°C. 



(2) Strain out the meat and add 5.0 g. 

 NaCl to the fluid. 



(3) Chop the stomachs of 5 hogs into 

 small pieces. 



(4) Mix 200.0 g. of (3), 10.0 g. HCl (pure) 

 and 1000.0 cc. water at 50°C. 



(5) Allow to stand at 50°C. for 12 to 24 

 hours. 



(6) Heat at 100°C. (Time not spec- 

 ified). 



(7) Strain or filter thru a layer of ab- 

 sorbent cotton. 



(8) Heat the filtrate and make alkaline 

 when the temperature reaches about 

 80°C. 



(9) Filter the fluid thru paper to clarify. 



(10) .Mix 1 liter of (9) with 1 liter of (2). 



(11) Heatto70°C. 



(12) Filter thru paper. 



(13) Make alkaline (method not given). 

 Sterilization: Method not given. 



Use: Cultivation of diphtheria bacilli and 

 to.xin production. Author reported that 

 heating the medium decreased the ability 



