CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



331 



(b) Henneberg prepared a 10.0% yeast 

 infusion solution and added 2.0 to 

 5.0% of any desired carbohydrate, 

 alcohol, etc. 



(c) Bobilioff-Preisser added one of the 

 following materials to yeast infusion: 

 dextrin glucose 

 galactose maltose 

 levulose raffinose 

 lactose sucrose 



The media were sterilized in the 

 steamer. 



(d) MUller-Thurgau and Osterwalder pre- 

 pared the basal solution as follows: 



(1) Add water (amount not given) to 

 1000.0 g. pressed yeast and boil. 



(2) Filter. 



(3) Dilute the filtrate to 10 liters by the 

 addition of water. 



(4) Acidify by the addition of 1.0% 

 malic acid. (Do not add acid here 

 when adding an acid as a carbon 

 source.) 



(5) Distribute in 100.0 cc. lots. 



(6) Sterilize (Method not given). 



(7) Add one of the following materials 



Glucose 2.0% or 4.0% 



Levulose 1.5% or 4.0% 



Galactose 4.0% 



Sucrose 5.0% or 4.0% 



Maltose 4.0% 



Malic acid 4.5% or 2.0% 



Potassium malate 



(acid) 10.0% 



Ethyl malate 10.0% 



Calcium malate (acid) 10.0% 



[2.2% or 4.7% 

 Citric acid or 2.0% or 



I 4.0% 



Lactose 4.0% 



Raffinose 1.0% 



1-arabinose 15.0% 



Xylose 10.0% 



a-methyl glucoside. . . 2.0% 



Glycerin 1.0% 



ammonium malate . . . 10.0% 



(8) Sterilize once more (method not 

 given). 



The media were used to study fer- 

 mentation by Bacterium mnnnito- 

 poeum, Bacterium intermedium, 

 Bad. Gayoni and mannitol bacteria 

 from wine. The authors also used 

 the basal solution without any 

 additions. 



(e) Besson prepared the medium as 

 follows: 



(1) Boil 100.0 g. of beer yeast in 

 1000.0 cc. of water. 



(2) Filter thru paper. 



(3) Add 5.0% of glucose or sucrose. 



(4) Add a small amount of phosphoric 

 acid and then add sufficient lime 

 water to give a slightly alkaline 

 reaction. 



(5) Heat for 5 minutes at 116 to IH^C. 



(6) Filter. 



(7) Tube. 



(8) Sterilize at 115°C. 

 References: Henneberg (1903 p. 8), Bobi- 

 lioff-Preisser (1916 p. 387), Muller- 

 Thurgau and Osterwalder (1918 p. 2), 

 Besson (1920 p. 35). 



1142. Heinemann's Glucose Yeast Infusion 

 Solution 



Constituents: 



1. Water 2000.0 g. 



2. Yeast 1.0 lb. 



3. Dextrose (10.0 to 



15.0%) 200.0 to 300.0 g. 



Preparation : 



(1) Boil one pound of pressed yeast or 

 1 liter of washed yeast with 2 liters of 

 water for one hour. 



(2) Filter the solution until clear. 



(3) Do not adjust the reaction. 



(4) Dissolve 10.0 to 15.0% dextrose in (3). 

 Sterilization: Sterilize in the Arnold on 3 



consecutive days. 

 Use: General culture medium. 

 Reference: Heinemann (1905 p. 130). 



1143. Gassner's Lactose Yeast Infusion 

 Solution 



Constituents: 



1. Water 18,000.0 cc. 



2. Yeast, Brewers.... 10,000.0 cc. 



3. Lactose 0.3% or 0.5% 



Preparation : 



(1) Place about 10 liters of brewers yeast 

 in a flask and wash with water. 

 Allow to stand for 30 minutes and 

 pour off the liquid. 



(2) Repeat the washing process until the 

 wash water is no longer brown but 

 slightly turbid. Washing 5 times is 

 usually sufficient. 



