CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



333 



B. racens, B. Pasteurianum, B. aceli, 

 B. melanogenum and B. xylunum. 

 He reported that after 3 days gener- 

 ally there was little or no reaction 

 with Fehling's solution. 

 References: Korff (1898 p. 532), Syree 

 (1899 p. 12), Henneberg (1903 p. 8), Will 

 (1908 p. 387), Waterman (1913 p. 455). 



1145. Bottger's Nitrate Yeast Infusion 



Solution 



Constituents: 



1. Yeast water 1000.0 cc. 



2. Sucrose (10.0%) 100.0 g. 



3. KNOs 0.2, 0.5, 1.0, 2.0 or 4.0% 

 Preparation : 



(1) Preparation of dilute yeast water not 

 specified. 



(2) Add 10.0% cane sugar to (1). 



(3) Add 0.2, 0.5, 1.0, 2.0 or 4.0% KNO3 

 to (2). 



Sterilization: Not specified. 



Use: To study toxicity of nitrates. Author 



reported that the presence of nitrates had 



little effect. 

 Variant: Author used 0.17, 0.41, 0.83, 1.67 



or 3.33% NaNO, instead of KNO3. 

 Reference: Bottger (1921 p. 224). 



1146. Janke's Alcohol Yeast Infusion 



Solution 



Constituents: 



1. Water 900.0 cc. 



2. Yeast (pressed) 100.0 g. 



3. Alcohol (3.0%) 30.0 cc. 



Preparation : 



(1) Boil 100.0 g. of pressed yeast with 

 900.0 cc. water. 



(2) Filter. 



(3) Add 3.0% by volume of absolute alco- 

 hol after sterilization. 



Sterilization: Method of sterilization of 



(2) not given. 

 Use: Cultivation of acetic acid bacteria 



found in beers. 

 Reference: Janke (1916 p. 6). 



1147. Bertrand's Sorbitol Yeast Infusion 

 Solution 



Constituents: 



1. Yeast infusion 1000.0 cc. 



2. KH2PO4 0.1 g. 



3. Sodium phosphate 0.1 g. 



4. CaClj 0.1 g. 



5. MgS04 0.06 g. 



6. Sorbitol (5.0%) 50.0 g. 



Preparation : 



(1) Preparation of yeast infusion not 

 given. It should contain about 5.0 g. 

 of dissolved material per liter. 



(2) Dissolve 2, 3, 4 and 5 in (1). 



(3) Add 5.0% sorbitol to (2). 

 Sterilization: Not specified. 



Use: Production of sorbose by Bacterium 



xylinum, Mycoderma aceli. 

 Reference: Bertrand (1898 p. 397). 



1148. Thoinot and Masselin's Yeast 

 Infusion Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Yeast 100.0 g. 



Preparation : 



(1) Add 100.0 g. of yeast to 1000.0 cc. of 

 water. 



(2) Mi.x slowly. 



(3) Boil in the autoclave or on a water 

 bath. 



(4) Make slightly alkaline by the addi- 

 tion of soda. 



(5) Filter. 



(6) Distribute as desired. 

 Sterilization: Sterilize in the autoclave at 



115° for 15 minutes. 

 Use: General culture medium. 

 Variants : 



(a) Henneberg used a 2.5, 5.0 or 10.0% 

 yeast water solution but did not give 

 the method of preparation. He culti- 

 vated lactic acid bacteria. 



(b) Heinemann prepared the medium 

 as follows: 



(1) Boil one pound of pressed yeast or 

 one liter of washed yeast with 

 2 liters of water for one hour. 



(2) Neutralize to phenolphthalein. 



(3) Filter the solution until clear. 



(4) Sterilize in the Arnold on 3 con- 

 secutive days. 



(c) Gassner prepared a similar medium 

 as follows: 



(1) Place about 10 liters of brewers 

 yeast in a flask and wash with 

 water. Allow to stand for 30 min- 

 utes and pour off the liquid. 



(2) Repeat the washing process until 

 the wash water is no longer brown 



