CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



335 



Sterilization: Sterilize thoroly (method not 



given). 

 Use: Decomposition of organic nitrogen 



products by Bacillus mycoides. 

 Reference: Stoklasa (1911 p. 470). 



1150. Lanken and Meyer's Fungus Infusion 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Fungus, dry 25.0 g. 



3. NaCl 5.0 g. 



Preparation : 



(1) Gather the fungus in large amounts 

 and remove all dirt from them. 



(2) Run thru a meat grinding machine. 



(3) Dry the chopped fungus in flat plates 

 and then rub to a fine powder which 

 is dried and stored. 



(4) Add 25.0 g. of (3) to 1000.0 g. water 

 and allow to stand at room tempera- 

 ture for 24 hours. 



(5) Filter. The filtrate should be clear. 

 If not, add a teaspoonful of filter 

 cell, shake thoroly and filter again, 



(6) Add 5.0 g. NaCl, and make alkaline 

 to litmus by the addition of 10.0% 

 soda solution standing for 20 minutes. 



Sterilization: Sterilize on 3 successive days 

 for ^ hour in the steamer. 



Use: Substitute for meat extract medium. 

 Authors used anthrax staphylococci, 

 colon-typhoid group and pyocyaneus, and 

 reported better growth than on media 

 containing peptone. Lactarius turpis, 

 Mordschwamm, Lactarius turminosus 

 white Reizker, Lactarius rufus Riibling 

 and Lactarius vuleruis Wolfschwamm were 

 the fungi used. 



Variants : 



(a) Wiegert prepared a similar medium 

 as follows: 



(1) Boil 50.0 g. dried fungus with 

 500.0 cc. water until the fungus is 

 softened. 



(2) Add 2.0 g. of NaCl. 



(3) Evaporate until a thick extract is 

 formed. 



(4) Add 1.0, 2.0 or 3.0% of this extract 

 instead of meat extract in the 

 preparation of nutrient media. 



(b) Klimmer used 50.0 g. dried fungus 

 instead of 25.0 g. 



References: Lanken and Meyer (1921 

 p. 511), Wiegert (1922-23 p. 110), Klim- 

 mer (1923 p. 204). 



1151. Ampola and Garino's Nitrate Peat 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Peat 20.0 g. 



3. NaNOs 3.2 g. 



Preparation: (1) Mix 2 and 3 with 1. 

 Sterilization: Method not given. 



Use: To study denitrification. Authors 

 reported that the nitrates were not 

 reduced. 



Reference: Ampola and Garino (1897 

 p. 309). 



1152. Revis' Peat Infusion Solution 



Constituents: 



1. Ammonium hydrate. 



2. Peat. 



3. CaCOa. 

 Preparation: 



(1) Exhaust peat with dilute ammonium 

 hydrate (Details of method not 

 given). 



(2) Neutralize the alkaline fluid with 

 phosphoric acid. 



(3) Filter and tube. 



(4) Add CaCOs (amount not given). 

 Sterilization: Not specified. 



Use: To study stability of physiological 



properties. 

 Reference: Revis (1910 p. 175). 



1153. Brussoff's Iron Peat Infusion Solution 



Constituents: 



1. Water. 



2. Peat. 



3. Iron. 

 Preparation : 



(1) Pour water (amount not given) over 

 finely divided peat in a beaker. 



(2) Heat in the autoclave under 2 atmos- 

 pheres pressure for 30 minutes. 



(3) Pour off the dark brown extract and 

 press the peat free from liquid. 



(4) Distribute the extract in high cylin- 

 drical glasses to a depth of 4 or 5 cm. 



(5) After adding a suitable amount (exact 

 amount not specified) of water, cover 

 the glasses with glass plate cover 

 gls 



