CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



339 



(11) Add 20.0 g. sucrose. 



(12) Distribute into test tubes. 



(13) Sterilize in the steamer or auto- 

 clave. 



References: Maze (1897 p. 45), Tanner 

 (1919 p. 58), Besson (1920 p. 35), Dopter 

 and Sacquepee (1921 p. 121), Harvey 

 (1921-22 p. 119). 



1163. de Rossi's Glucose Bean Infusion 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Beans 10.0% 100.0 g. 



3. Glucose 2.0% 20.0 g. 



Preparation : 



(1) Prepare a bean infusion using 10.0% 

 beans. 



(2) Dissolve 2.0% glucose in (1). 

 Sterilization: Not specified. 



Use: To study nitrogen fixation by bacteria 



from nodules of leguminous plants. 

 Reference: de Rossi (1909), (1910 p. 271). 



1164. Kaufmann's Jequirity Infusion 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Jequirity seeds 100.0 g. 



Preparation : 



(1) Grind 100.0 g. Jequirity seeds in a 

 mortar. 



(2) The peeled or shelled seeds now 

 weight about 80.0 g. 



(3) Add to 1000.0 cc. of water. 



(4) Boil in a steam sterilizer for about 

 two hours. 



(5) Cool and filter. 



(6) Reaction is neutral or slightly alka- 

 line. 



(7) Distribute into test tubes. 

 Sterilization: Sterilize in the usual manner 



(method not given). 



Use: General culture medium. Author re- 

 ported that the medium supported the 

 growth of Bacillus pyocT/aneus and many 

 other organisms. 



Variants: The author aded 6.0% glycerol 

 or 6.0% glycerol with 2.0% peptone. 



Reference: Kaufmann (1891 pp. 65, 68). 



1165. Tanner's Pea Flour Infusion Solution 



Constituents: 

 1. Distilled water 1000.0 cc. 



2. Pea flour 100.0 g. 



3. NaCl 100.0 g. 



Preparation : 



(1) Add 100.0 g. of pea flour and 100.0 g. 

 NaCl to 1 liter distilled water. 



(2) Mix and steam, stirring constantly 

 for 30 minutes. 



(3) Allow to settle. 



(4) Filter. 



Sterilization: Method not given. 



Use: Peptone substitute. The author re- 

 ported that this solution was especially 

 designed to replace Witte's peptone. 

 The infusion should, preferably, be made 

 fresh each time it is used. 



Reference: Tanner (1919 p. 57). 



1166. Stutzer's Basal Legume Seed Infusion 

 Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Pea seeds 10.0 g. 



or 

 clover seeds {Trifol hy- 

 bridum) 10.0 g. 



or 

 bean seeds (Vicia Faba) 10.0 g. 



or 



lupine seeds 10.0 g. 



Preparation : 



(1) Grind pea seeds, clover seeds {Trifol 

 hybridum), bean seeds {Vicia Faba) 

 or lupine seeds and pour 1 liter of 

 water over each 10.0 g. of one of the 

 ground seeds. 



(2) Boil for 1 to 2 hours, adding the water 

 lost by evaporation. 



(3) Filter. 



(4) Dilute or concentrate the filtrate as 

 desired so that the ratio of pea seed 

 to water be 100, 75, 50, 40, 30, 20, 10, 

 5, 4, 3, 2 or 1.0 g. per liter. Best 

 results are obtained however using 

 10.0 g. peas per liter water as in (1). 



(5) Dissolve one of the added nutrients 

 in (4). 



Sterilization: Not specified. 



Use: The study the cultivation of Pisum 

 sativum, Vica Faba, Trifoliuin pratense 

 and Trifolium incarnatum. Author re- 

 ported that generally the addition of 

 carbohydrates and asparagin favored the 

 growth of the bacteroids. Oil seemed to 

 have little effect. 



