340 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Added nutrients and variants: 



(a) The author added one of the fol- 

 lowing : 



Asparagin 2.0% 



Glucose 1.0, 5.0 or 10.0% 



Sucrose 1.0, 5.0 or 10.0% 



starch 2.0% 



flour 2.0% 



inulin 2.0% 



oil small amount 



(b) The author prepared an infusion from 

 clover seeds in the following manner: 



(1) Heat 3.0 to 4.0 g. of clover seed in 

 water on a water bath for several 

 hours. 



(2) Pulverize the soft seeds in a rub- 

 bing dish. 



(3) Heat again for an hour. 



(4) Add distilled water until 1000.0 cc. 

 are obtained (1.0 g. K2HPO4 may 

 be added). 



(5) Distribute in 10.0 cc. lots. 



(6) Sterilize 3 times in the usual way. 

 (Method not given.) 



(c) The author added 0.1% K2HPO4 to an 

 infusion prepared as indicated in the 

 basic solution using 2.5 g. Vicia Faha 

 per liter and added 0.0 or 1.0 g. 

 K0HPO4. 



(d) The author used the basic solution 

 without any additions. 



Reference: Stutzer (1900 pp. 898, 903, 908). 



1167. Smith's Potato Infusion Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Potato 500.0 g. 



Preparation : 



(1) Pass clean, pared potatoes rapidly 

 thru a grating machine. 



(2) Throw immediately into double the 

 weight of distilled water. 



(3) Place on a water bath and quickly 

 raise the temperature to 55°C. Keep 

 at this temperature for an hour, stir- 

 ring frequently. 



(4) Filter. 



Sterilization: Sterilize in the steamer. 

 Use: General culture medium. Author 



cultivated plant parasites. 

 Variants : 



(a) Peklo cultivated plant actinomyces 

 on a medium prepared as follows: 

 (1) Prepare a pulp from raw potatoes. 



(2) Add a little distilled water (amount 

 not given). 



(3) Allow to stand in the cold for 

 24 hours. 



(4) Press thru a towel. 



(5) Sterilize until there is no more co- 

 agulum formed (time not specified). 

 (Filtration not specified.) 



(b) Tanner prepared a similar medium as 

 follows: 



(1) Wash and grind a few potatoes. 



(2) To 30.0 g. of (1) add a liter of dis- 

 tilled water. 



(3) Allow to stand in the ice box over 

 night. 



(4) Filter. 



(5) Boil the filtrate. 



(6) Filter again if necessary. 



(7) Tube. 



(8) Sterilize (method not given). 



(9) If the reaction is too acid, adjust 

 as usual. 



(c) Besson prepared the medium as 

 follows: 



(1) Clean and grate potatoes. 



(2) Soak 30.0 g. of (1) in 1000.0 cc. 

 water for 4 hours. 



(3) Decant the liquid. 



(4) Boil. 



(5) Filter. 



(6) Reaction may be neutralized if 

 desired. 



(7) Distribute as desired. 



(8) Sterilize in the autoclave or 

 steamer. 



(d) Dopter and Sacquepee prepared a 

 similar medium as follows: 



(1) Grate potatoes. 



(2) Macerate 10.0 to 20.0 g. of (1) with 

 1 liter of water for two hours. 

 (Temperature not specified.) 



(3) Strain thru a cloth. 



(4) Bring to a boil. 



(5) Filter thru paper. 



(6) Distribute. 



(7) Sterilize in the autoclave. 



(e) Robertson and Davis studied the 

 influence of vitamines on bacterial 

 growth. They reported that the me- 

 dium did not give continued growth 

 of yeast. The medium was prepared 

 as follows: 



(1) Grate potato fine and cover with 

 sterile distilled water. 



