CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



341 



(2) Allow to stand at room tempera- 

 ture for 18 hours. 



(3) Pass thru a Berkefeld filter. 



(4) Adjustment of reaction not given. 



(5) Add sterile (method of sterilization 

 not given) physiological salt solu- 

 tion to (3). 



(f) Park, Williams and Krumwiede pre- 

 pared the medium as follows: 



(1) Run white potatoes thru the grater 

 or chopping machine. 



(2) Mix 1.0 pound of (1) with water. 



(3) Soak over night. 



(4) Heat to boiling. 



(5) Press thru cheese cloth. 



(6) Add one egg per liter. 



(7) Autoclave one hour. 



(8) Filter thru cotton (very tedious). 



(9) Store in flasks and sterilize in the 

 autoclave for 30 minutes at 15 

 pounds pressure. 



References: Smith (1905 p. 42), Peklo (1910 

 p. 551), Tanner (1919 p. 60), Besson (1920 

 p. 35), Dopter and Sacquepee (1921 

 p. 120), Robertson and Davis (1923 p. 154), 

 Park, Williams and Krumwiede (1924 

 p. 122). 



1168. Eisner's Hydrochinone Potato 



Infusion Solution 



Constituents: 



1. Potato water. 



2. Hydrochinone. 

 Preparation : 



(1) Preparation of potato water not 

 given. 



(2) Do not adjust the reaction. 



(3) Add hydrochinone, amount not given. 

 Sterilization: Method not given. The me- 

 dium turns brown during sterilization. 



Use: Differentiation of coli and typhoid 

 bacteria. Author reported that B. coli 

 reduced the brown color. Typhoid bac- 

 teria gave no color change. 



Reference: Eisner (1896 p. 28). 



1169. Robertson and Davis' Potato 



Infusion Solution 



Constituents: 



1. Sterile distilled water 1000.0 cc. 



2. Asparagin (Merck) 3.4 g. 



3. CaClj 0.1 g. 



4. Glucose 20.0 g. 



5. MgS04 0.2 g. 



6. K2HPO4 1.0 g. 



7. NaCl 5.0 g. 



8. Potato 

 Preparation: 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1 by 

 boiling 3 minutes. 



(2) Restore original volume with sterile 

 distilled water. 



(3) Adjust reaction to pH = 7.4. 



(4) Tube. 



(5) Grate potato fine and cover with 

 sterile distilled water. 



(6) Allow to stand at room temperatures 

 for 18 hours. 



(7) Pass thru a Berkefeld filter. 



(8) Add various amounts of (7) to each 

 tube of sterile (4). 



Sterilization: Autoclave (3) at 20 pounds 



pressure for 30 minutes. 

 Use: To study influence of vitamines on 



bacterial growth. Authors reported that 



yeast grew very luxuriantly. 

 Reference: Robertson and Davis (1923 



p. 154). 



1170. Lubinski's Glycerol Potato 

 Infusion Solution 



Constituents: 



1. Water 1500.0 cc. 



2. Potato 1000.0 g. 



3. Glycerol (4.0%) 60.0 g. 



Preparation : 



(1) Wash potatoes clean and cut them in 

 small pieces. 



(2) Boil (1) for 3 or 4 hours under a free 

 flame, or in a steamer, in 1500.0 cc. 

 of water. 



(3) Filter. 



(4) Add 4.0% glycerol to the filtrate. 



(5) Neutralize (may be used without 

 neutralization). 



Sterilization: Method not specified. 

 Use: Cultivation of tubercle bacilli. 

 Variants : 



(a) Harvey prepared the medium as 

 follows: 



(1) Grate finely washed peeled po- 

 tatoes. 



(2) Add 1000.0 to 2000.0 cc. water. 



(3) Heat the mixture 20 minutes to a 

 temperature not exceeding 50°C. 



(4) Raise to boiling temperature. 



(5) Boil 10 minutes. 



