342 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(6) Pour the mixture on a clean, thick 

 cloth. 



(7) Collect the fluid which drains thru 

 the cloth together with that ob- 

 tained by squeezing the cloth. 



(8) Filter the fluid collected thru 

 thick filter paper. 



(9) Add the filtrate to an equal quan- 

 tity of distilled water. 



(10) Steam 60 minutes. 



(11) Add glycerol to 4 per cent. 



(12) Mix well. 



(13) Filter. 



(14) Distribute into test tubes. 



(15) Sterilize in the autoclave or 

 steamer. 



(b) Harvey prepared a medium similar 

 to the above as follows: 



(1) Grate finely washed, peeled po- 

 tatoes. 



(2) Add 2 parts 4 per cent glycerol to 

 1 part potato gratings. 



(3) Boil. 



(4) Filter. 



(5) Use the filtrate as potato extract. 

 References: Lubinski (1895 p. 126), Harvey 



(1921-22 pp. 118, 119). 



1171. Berthelot's Vegetable Infusion 

 Solution 



Constituents: 



1. Water 4000.0 cc. 



2. Potato 300.0 g. 



3. Carrots 150.0 g. 



4. Turnips 150.0 g. 



Preparation : 



(1) Peel the potatoes. They may be 

 left whole or cut in two. 



(2) Wash the carrots and turnips. 



(3) Mix (1) and (2) in 4 liters of cold 

 water. 



(4) Bring to boil and boil for 4 hours in 

 order to reduce the volume of water 

 one-fourth. 



(5) Filter thru a fine towel. 



(6) Bring the filtrate to 3 liters volume 

 either by further boiling or adding 

 distilled water. 



(7) Add 10.0% soda solution to neutral- 

 ize boiling (6). The reaction may 

 be adjusted to a slight alkalinity to 

 litmus. 



(8) Heat in the autoclave at 120° for 

 30 minutes. 



(9) Allow to stand for 24 hours in the 

 cold or better on ice. 



(10) Filter thru paper. 



(11) Distribute as desired. 

 Sterilization: Sterilize at 115° for 20 



minutes. 



Use: General culture medium for sapro- 

 phytes and pathogenic forms. 



Variants: Tanner gave the following 

 method of preparation: 



(1) Peel the potatoes. 



(2) Wash the carrots and turnips. 



(3) Cut (1) and (2) in small pieces and 

 place in cold water. 



(4) Boil 4 hours. 



(5) Strain. 



(6) Make weakly alkaline. 



(7) Heat in autoclave at 120°C. 



(8) Allow to stand in a refrigerator 

 over night. 



(9) Filter thru paper. 



(10) Final sterilization not given. 

 References: Berthelot (1917 p. 131), Tan- 

 ner (1919 p. 46). 



1172. Migula's Potato Juice 



Constituents: 



1. Potato. 

 Preparation: 



(1) Pass peeled potatoes thru a sieve, or 

 grind real fine. 



(2) Press the juice thru a linen towel. 



(3) Distribute in tubes. 

 Sterilization : Boil for 20 minutes on each of 



3 successive days in a steamer. 

 Use: General culture medium. 

 Reference: Migula (1901 p. 20). 



1173. Winogradsky's Gypsum Root Solution 



Constituents: 



1. Water 3000.0 to 5000.0 cc. 



2. Gypsum 



3. "Butomus-Rhizonus" 

 Preparation : 



(1) Cut a "Butomus-Rhizomus," freshly 

 removed from the ground, into several 

 pieces. 



(2) Place the pieces containing mud or 

 slime into a deep jar, containing 3.0 

 to 5.0 liters of water. 



(3) Add a few grams of gypsum. 

 Sterilization: Not specified. 



Use: Enrichment of sulphur bacteria. 

 Author reported that H2S was formed 



