CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



343 



after 5 to 7 days. The slime at the bot- 

 tom became black. The liquid became 

 opalescent and surface 3 to 6 weeks a 

 membrane of sulfur formed on the surface. 

 Reference: Winogradsky (1888 p. 11). 



1174. Tanner's Carrot Infusion Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Carrots 30.0 g. 



Preparation : 



(1) Wash and grind a few carrots. 



(2) To 30.0 g. of (1) add a liter of dis- 

 tilled water. 



(3) Allow to stand in the ice box over 

 night. 



(4) Filter. 



(5) Boil the filtrate. 



(6) Filter again if necessary. 



(7) Tube. 



(8) If the reaction is too acid, adjust 

 as usual. 



Sterilization: Method not given. 

 Use: General culture medium. 

 Variants : 



(a) Besson soaked the carrots for 4 hours 

 instead of over night, as in step (3) 

 above. 



(b) Dopter and Sacquepee prepared the 

 medium as follows: 



(1) Grate carrots. 



(2) Macerate 10.0 to 20.0 g. of (1) with 

 1 liter of water for 2 hours. (Tem- 

 perature not specified.) 



(3) Strain thru a cloth. 



(4) Bring to a boil. 



(5) Filter thru paper. 



(6) Distribute. 



(7) Sterilize in the autoclave. 



(c) Robertson and Davis studied the 

 influence of vitamines on bacterial 

 growth. They prepared a medium 

 as follows: 



(1) Grate carrots fine, and cover with 

 sterile distilled water. 



(2) Let stand at room temperatures for 

 18 hours. 



(3) Pass thru a Berkefeld filter. 



(4) Adjustment of reaction not given. 



(5) Add to (3) sterile (method of 

 sterilization not given) physio- 

 logical salt solution. Amounts not 

 specified. 



They reported that the medium did 

 not give continued growth of yeast. 



(d) Draper gave the following method of 

 preparation. The medium was used 

 for the cultivation of Oidium albi- 

 cans. 



(1) Chop raw carrots into fine particles 

 and allow to extract in sterile dis- 

 tilled water at about 10°C. for 

 48 hours. 



(2) Filter. 



(3) Tube. 



(4) Autoclave for 15 minutes at 15 

 pounds pressure. 



References: Tanner (1919 p. 60), Besson 

 (1920 p. 35), Dopter and Sacquepee (1921 

 p. 120), Robertson and Davis (1923 

 p. 154), Draper (1924 p. 635). 



1175. Robertson and Davis' Carrot Infusion 

 Solution 



Medium identical with medium 1169 but 

 carrot used instead of potato. 



1176. Migula's Hay Infusion Solution 



Constituents : 



1. Water 3000.0 cc. 



2. Hay 100.0 g. 



Preparation : 



(1) Pour 3 liters of water over 100.0 g. of 

 hay and allow to stand for 24 hours 

 in a warm place. 



(2) Press thru linen. 



(3) Neutralize and make slightly alkaline 

 to litmus. 



(4) Boil for one hour. 



(5) Cool to 40°C. and add the whites of 

 3 eggs. 



(6) Boil and filter. 



(7) Distribute in 100.0 cc. lots in flasks. 

 Sterilization: Steam on 3 successive days 



for one hour each day. 



Use: General culture medium. 



Variants: The following methods of prep- 

 aration have been suggested by various 

 investigators : 



(a) Heinemann. 



(1) Macerate 10.0 g. of chopped hay in 

 1000.0 g. water in the water bath 

 for 3 hours. 



(2) Filter. 



(3) Sterilize in the autoclave for 10 

 minutes at 120°C. 



(b) Linde cultivated Cladothrix in a 

 light yellow completely transparent 

 hay infusion, or in a coffee brown 

 hardly transparent medium. The 



