344 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



media were employed without any 

 additions, or made slightly alkaline 

 by the addition of NaoCOs. 



(c) Tausz and Peter. 



(1) Wash grass that has been piled up 

 for a few days and only slightly 

 decomposed, with a little water to 

 remove the dirt. 



(2) Boil with a little water. 



(3) Cut the grass into small pieces and 

 boil for 20 minutes with 3.5 liters 

 of water, 



(4) Filter. The filtrate is dark brown. 



(5) Neutralize by the addition of sev- 

 eral drops of KOH. 



(6) Sterilize by steaming on each of 3 

 successive days for 30 minutes. 



They cultivated Bacterium alipha- 

 ticum, Bacterium aliphaticum lique- 

 faciens, paraffin bacteria. 



(d) Tanner, Besson, and Dopter and 

 Sacquepee. 



(1) Macerate 15.0 to 20.0 g. of finely 

 chopped hay in 1000.0 g. of water 

 for one or two hours. 



(2) Boil a few minutes. 



(3) Filter. 



(4) Tube. 



(5) Sterilize at 115°C. 



(6) The infusion may be neutralized if 

 the reaction is a little acid. 



Besson, and Dopter and Sacquepee 

 treated straw in exactly the same 

 manner, 

 (e) Harvey. 



(1) Prepare dried chopped hay 10; dis- 

 tilled water 1000, in a well-stop- 

 pered bottle. 



(2) Heat to 70°C. 



(3) Close tightly with stopper. 



(4) Heat 3 hours at 60°C. on a water 

 bath, 



(5) Steam 60 minutes. 



(6) Filter thru thick filter paper. 



(7) Distribute into test tubes. 



(8) Sterilize in the autoclave or 

 steamer. 



(f) Harvey. 



(1) Mince finely hay or straw. 



(2) Add 15 to 20.0 g. to 1000.0 cc. water. 



(3) Heat the mixture at a temperature 

 not exceeding 50°C. 



(4) Raise the temperature rapidly to 

 boiling point. 



(5) Boil 10 minutes, 



(6) Adjust reaction, 



(7) Filter. 



(8) Distribute into flasks or test tubes. 



(9) Sterilize in the autoclave or 

 steamer. 



References: Migula (1901 p. 20), Heine- 

 mann (1905 p. 129), Linde (1913 p. 386), 

 Tausz and Peter (1919 p. 509), Tanner 

 (1919 p. 57), Besson (1920 p. 35), Dopter 

 and Sacquepee (1921 p. 120), Harvey 

 (1921-22 pp. 120, 121). 



1177. Winogradsky's Ferric Hydroxide Hay 

 Infusion Solution (Molisch) 



Constituents: 



1. Water. 



2. Hay. 



3. Ferric hydroxide. 

 Preparation : 



(1) Place a handful of macerated hay 

 that has been well extracted with 

 water in a glass cylinder 50 cm. high. 



(2) Sprinkle some freshly precipitated 

 ferric hydroxide on the hay, 



(3) Fill the cylinder with well water. 

 Sterilization: Not specified. 



Use: Cultivation of iron bacteria, Chlamy- 



dothrix {Leptothrix) ochracea. 

 Reference: Molisch (1910 p. 32). 



1178. Jensen's Nitrate Straw Solution 



Constituents: 



1. Water 100.0 cc. 



2. Straw 5.0 g. 



3. NaN03 1.0 g. 



Preparation: (1) Mix 2 and 3 with 1. 

 Sterilization: Not specified. 



Use: To study denitrification by straw 

 forms. The author reported that glycerol 

 aided in the reduction of nitrates. 



Variants: The author added 2.0% glycerol. 



Reference: Jensen (1897 p. 691). 



1179. Wolbach and Singer's Glucose Hay 

 Infusion Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Hay 120.0 g. 



3. Glucose 10.0 g. 



Preparation : 



(1) Cut 120.0 g. hay fine and soak in 1 

 liter of water for | hour. Final reac- 



