CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



345 



tion being about 0.2% acid to plienol- 

 phthalein. 



(2) Decant the water and add 1.0% 

 glucose. 



(3) Adjust the reaction to neutral to 

 phenolphthalein. 



(4) Tube after sterilization. 

 Sterilization: Sterilize by filtration thru 



Chamberland F filter. 



Use: Cultivation oi Spirocheta elusa (free 

 living). Author reported that the me- 

 dium was clouded by growth in about 

 48 hours. Medium not so satisfactory if 

 sterilized by repeated steaming or in the 

 autoclave. 



Reference: Wolbach and Binger (1914 p. 9). 



1180. Sherman's Soil Hay Infusion Solution 



Constituents: 



1. Distilled water 900.0 cc. 



2. Soil 100.0 g. 



3. Hay infusion (1.0%) 190.0 cc. 



4. CaCOs small excess 



Preparation : 



(1) Boil 1 and 2. 



(2) Filter clear. 



(3) Add 3. 



(4) Add 4 in small excess. 

 Sterilization: Method not given. 



Use: Cultivation of protozoa, flagellates, 



ciliates and amoebae. 

 Reference: Sherman (1916 p. 42). 



1181. Smith's Cabbage Infusion Solution 



Constituents: 



1. Distilled water. 



2. Cabbage leaves. 



Preparation: (1) Boil a few cabbage leaves 

 in distilled water. 



Sterilization: Method not given. 



Use: Cultivation of Pseudomonas cam- 

 pestris (Pammel). Author reported that 

 organisms grew very well in the medium 

 producing a heavy clouding and a copious 

 yellow precipitate. B. cloacae gave gas 

 from this medium. In litmus medium, 

 the culture continued to be red for several 

 days. Then the litmus lost its color ex- 

 cept in the very top layer where it came 

 in contact with air. 



Variants: The author added sufficient 

 Trommsdorf's litmus to color the solution 

 a pale red. Re-sterilize. 



Reference: Smith (1897 p. 479). 



1182. Conrad's Glucose Cabbage Infusion 



Solution 

 Constituents: 



1. Water 200.0 cc. 



2. Cabbage 100.0 g. 



8. Glucose 3.0% 



4. NaCl 0.5% 



Preparation: 



(1) Prepare an extract of cabbage (Weiss- 

 kraut abkochung) in the ratio of 1:2. 

 Method not given. 



(2) Add 3.0% glucose and 0.5% NaCl 

 to(l)._ 



(3) Place in a large flask. 

 Sterilization: Sterilize on 3 successive days 



in the steamer. 

 Use : To study fermentation by sauerkraut 



bacteria {Bacterium brassicae acidae). 

 Reference: Conrad (1897 p. 65). 



1183. Will's Cabbage Juice 



Constituents: 



1. Cabbage juice. 



Preparation: (1) Method of obtaining cab- 

 bage juice not given. 



Sterilization: Method not given. 



Use: Cultivation of mycoderma. Fred and 

 Peterson studied the effect of different 

 NaCl concentrations on the growth of 

 lactic acid producing bacteria. They re- 

 ported that the higher the salt concentra- 

 tion the less acid produced, 4.0% was the 

 critical point. Yeast grew well at 4.0% 

 or higher NaCl concentrations, m ' 



Variants: Fred and Peterson added 0.0, 1.0, 

 2.0, 4.0, 5.0 or 6.0% NaCl to cabbage 

 juice. 



References : Will (1910 p. 3), Fred and Peter- 

 son (1922 p. 266). 



1184. Reed and Cooley's Basal Spinach 

 Infusion Solution 

 Constituents: 



1. Water 500.0 cc. 



2. Spinach 100.0 g. 



Preparation : 



(1) Steep 100.0 g. of young spinach leaves 

 in 500.0 cc. water. 



(2) Filter. 



(3) Neutralize. 



(4) Add H2SO4 to make slightly acid to 

 litmus. 



(5) Dissolve 1.0% of one of the added 

 nutrients in (4). 



