346 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Sterilization: Not specified. 



Use: Cultivation of Heterosporium vari- 



ahile. 

 Added nutrients: The author added 1.0% 



NH4NO3 or 1.0% peptone. 

 Reference: Reed and Cooley (1911-12 



p. 50). 



1185. Sucksdorff' s Tea Infusion 



Constituents : 



1. Water 1000.0 cc. 



2. Tea 10.0 g. 



Preparation : 



(1) Place 10.0 g. tea in a liter of boiling 

 water. 



(2) After 5 minutes heating filter half the 

 tea into a clean flask. Continue heat- 

 ing the other half for 5 minutes and 

 then filter into a clean flask. 



(3) Distribute into clean non-sterile 

 beakers and place in an open window. 



Sterilization: Do not sterilize. 



Use: To study the type of organisms that 

 will grow in tea. The author reported 

 that schizomycetes grew better in tea 

 than coffee. Molds, however, grow bet- 

 ter in coffee (1192) than tea. 



Reference: Sucksdorff (1886 p. 368). 



1186. Bushnell's Asparagus Solution 



Constituents : 



1. Tap water 300.0 cc. 



2. Asparagus 100.0 g. 



Preparation: (1) Suspend asparagus in 



water (method not given). 

 Sterilization: Autoclave at 20 pounds for 



30 minutes. 

 Use: To study changes (chemical) produced 



by anaerobes, B. sporogenes group. 

 Reference: Bushnell (1922 p. 381). 



1187. Tausz and Peter's Leaf Infusion 

 Solution 



Constituents: 



1. Water 3500.0 cc. 



2. Leaves, decomposed 70.0 g. 



Preparation : 



(1) Boil 70.0 g. of decomposed leaves in 

 3.5 liters of water for about 40 min- 

 utes. 



(2) Filter. 



(3) Neutralize by the addition of several 

 drops of KOH. 



(4) Distribute in flasks and plug. 



Sterilization: Sterilize by steaming for 30 



minutes on each of 3 successive days. 

 Use: Cultivation of Bacterium alipha- 



ticum, Bacterium aliphaticum liquefaciens, 



paraffin bacteria. 

 Variants : The author gave the following 



method of preparation. 



(1) Pour boiling water over 150.0 g. fresh 

 green maple leaves and then cut into 

 small pieces. 



(2) Boil with 3.5 liters of water for 

 30 minutes. 



(3) Filter or clarify by allowing the ma- 

 terial to stand. The filtrate is 

 green. Filtration proceeds slowly. 



(4) Neutralize by the addition of KOH 

 until phenolphthalein is colored 

 slightly red. 



(5) Flask and plug. 



(6) Sterilize by steaming for 30 minutes 

 on each of 3 successive days. 



Reference: Tausz and Peter (1919 p. 49). 



1188. Miiller-Thurgau's Grape Vine 

 Infusion Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Grape vine (stems) 100.0 g. 



3. Sucrose 50.0 g. 



Preparation: 



(1) Prepare an infusion of 100.0 g. of 

 grape vine (stems) in 1000.0 cc. of 

 water, method not given. 



(2) Add 5.0% sucrose to (1). 



(3) Dissolve 1.0%, Ca3(P04)2 and 0.5% 

 MgS04 in (2). 



Sterilization: Method not given. 



Use: Cultivation of red burning fungus of 

 grape vines. Pseudopeziza tracheiphila 

 Author reported that fungus grew luxu- 

 riant at first; then began to turn brown 

 and to show different degeneration 

 products. 



Variants: The author omitted the 

 Ca3(P04)2 and MgS04 and added 5.0% 

 bouillon. 



Reference: Miiller-Thurgau (1903 p. 82). 



1189. Malenkovic's Wood Infusion Solution 



Constituents : 



1. Water 1000.0 cc. 



2. KNOa...., 1.5 g. 



3. K:HP04 1.5 g. 



4. NH4H2PO4 1.0 g. 



