CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



347 



5. MgS04 0.5 g. 



6. Wood 

 Preparation: 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Extract 1000.0 cc. wood with (I). 

 (Method not given.) 



Sterilization: Not specified. 



Use: Cultivation of Coniophora cerebella 



{Corticium putaneum). Author reported 



good growth. 

 Reference: Malenkovic (1906 p. 409). 



1190. Olitsky's Tomato Infusion 



Constituents: 



1. Distilled water 250.0 cc. 



2. Tomato plants 80.0 g. 



Preparation : 



(1) Select 80.0 g. of fresh young stems, 

 leaves and shoots of a healthy tomato 

 plant. 



(2) Cut in small pieces and grind by hand 

 to a soft pulp in a large mortar. 



(3) Thoroughly mix with 250.0 cc. of 

 sterile distilled water, having a pH 

 between 5.3 and 5.4. 



(4) Centrifuge at a high speed for one 

 hour. 



(5) Medium discarded if not crystal 

 clear, or reaction does not lie within 

 5.3 and 6.0 on the Sorensen scale. 



Sterilization: Pass the supernatant fluid 

 through two sterile Berkefeld filters size 

 N. Incubate at 7 days at 28-30°C. 



Use: Cultivation of the active agent of 

 mosaic of tobacco and tomato. 



Reference: Olitsky (1925 p. 131). 



1191. Schardinger's Prune Solution 



Constituents: 



1. Water 200.0 cc. 



2. Prunes 500.0 g. 



3. CaCOs 10.0 g. 



Preparation: (1) Sterilize 200.0 cc. water, 



500.0 g. of pitted prunes and 10.0 g. 

 CaCOa. 



Sterilization: Given in step (1) above. 



Use: Cultivation of Bacillus ?nacerans and 

 used as a general culture medium. 



Variants: Tanner prepared a similar me- 

 dium as follows: 



(1) Macerate 50.0 g. of prunes in 1 liter 

 of distilled water. 



(2) Allow to stand in the ice chest until 

 next morning. 



(3) Filter. 



(4) Tube. 



(5) Sterilize in the autoclave or steamer. 

 References: Schardinger (1907 p. 161), Tan- 

 ner (1919 p. 57). 



1192. Sucksdorff's Coffee Infusion 

 Constituents: 



1. Water 1000.0 cc. 



2. Coffee 50.0 g. 



Preparation : 



(1) Pour 1 liter of boiling water over 

 50.0 g. coffee. 



(2) Boil for 2 to 3 minutes. 



(3) Filter thru a double filter into a clean 

 flask. 



(4) Distribute into clean non-sterile 

 beakers and place in an open window. 



Sterilization: Do not sterilize. 



Use: To study the type of organisms that 

 develop in coffee. Author reported that 

 molds grew well in the coffee, while 

 schizomycetes grew better in tea (1185). 



Reference: Sucksdorff (1886 p. 368). 



1193. Tanner's Fruit Infusion Solution 

 Constituents: 



1. Water 1000.0 cc. 



2. Fruit (dried) 50.0 to 100.0 g. 



Preparation : 



(1) Macerate 50.0 to 100.0 g. of dried 

 fruit in a liter of water for several 

 hours. 



(2) Stew (1) in the water (time not 

 given). 



(3) Pass thru a coarse sieve. 



(4) Boil. 



(5) Filter. 



(6) Tube. 



Sterilization: Sterilize at 115°C. 

 Use: Cultivation of molds. 

 Variants: Harvey used bananas, prunes or 

 raisins and proceeded as follows: 



(1) Mince finely bananas, prunes or 

 raisins. 



(2) Add 75.0 g. to 1000.0 cc. of water. 



(3) Heat the mixture 20 minutes at a 

 temperature not exceeding 50°C. 



(4) Raise the temperature to boiling 

 point. 



(5) Boil 10 minutes. 



(6) Strain the fluid thru a thick, clean 

 cloth and squeeze out the residue of 

 the fluid. 



