352 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



B. aceti, Acetobacter melanogenum, B. 

 xylinum. 

 Reference: Waterman (1913 p. 452). 



1212. Wurtz's Malt Infusion Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Malt. 100.0 g. 



Preparation : 



(1) Grind 103.0 g. of malt. 



(2) Add (1) to 1000.0 cc. of water. 



(3) Heat for one hour between 55-58°C. 

 (not above 58°). 



(4) Boil. 



(5) Filter. 



Sterilization: Sterilize at 115° for 15 



minutes. 

 Use: Cultivation of yeast, molds and 



trichophyta. 

 Variants : 



(a) Thoinot and Masselin prepared a sim- 

 ilar medium from malt dust as 

 follows: 



(1) Wash 100.0 g. of malt dust with 

 water. 



(2) Boil in a liter of water. 



(3) Filter. 



(4) Distribute as desired. 



(5) Sterilize at 115° for 15 minutes. 



(b) Tanner prepared a similar medium 

 as follows: 



(1) Place 250.0 g. of malt into a flask. 



(2) Add 1000.0 cc. of distilled water 

 heated to 70°C. to (1), and close 

 with a rubber stopper. 



(3) Place in a water bath at 60 °C. for 

 1 hour. 



(4) Strain thru cheese cloth into clean 

 flasks. 



(5) Heat in an Arnold for 30 minutes. 



(6) Filter thru paper. 



(7) Tube in 10.0 cc. quantities. 



(8) Sterilize in an Arnold. 



(c) Dopter and Sacquepee prepared a 

 medium in the same manner as vari- 

 ant (a) above, but used only 50.0 g. 

 of malt dust instead of 100.0 g. 



(d) Harvey cultivated yeast, molds and 

 trichophyta on a medium prepared 

 as follows: 



(1) Prepare: Crushed malt 1 part; dis- 

 tilled water at 70°C. 4 parts. 



(2) Keep 60 minutes at 60°C. in a flask 

 closed with a rubber cork. 



(3) Strain thru muslin. 



(4) Steam 30 minutes. 



(5) Filter. 



(6) Sterilize 20 minutes at 100°C., 

 3 days. 



(e) Harvey added 25.0 cc. of the solution 

 obtained from variant (d) to a liter 

 of water. Further preparation not 

 given. 



(f) Harvey added 100.0 g. of malt to a 

 liter of water. Further preparation 

 not given. 



References: Wurtz (1897 p. 46), Thoinot 

 and Masselin (1902 p. 30), Tanner (1919 

 pp. 57, 58), Bezan^on (1920 p. 115), Bes- 

 son (1920 p. 34), Dopter and Sacquepee 

 (1921 p. 120), Harvey (1921-22 pp. 112, 

 113). 



1213. Beijerinck's Malt Wort Solution 



Constituents : 



1. Water, ditch 1000.0 cc. 



2. Malt wort 3.0 cc. 



3. NasCOs 1.0 g. 



4. Mohr's salt (ammonium 

 ferrous sulphate) 0.2 g. 



Preparation: 



(1) Add 2, (about 10° Balling) 3, and 4 to 

 1 liter of ditch water. 



(2) A precipitate of calcium and ferro- 

 phosphate and carbonate forms. 



(3) Shake well so the precipitate is 

 thoroly distributed, and distribute 

 the mixture into a flask or a beaker. 

 Fill the flask full so that there is little 

 surface exposed to air or cover the 

 beaker with a tight fitting glass plate. 



(4) Place in the incubator about 25 to 

 30 °C. 



Sterilization: Not specified. 



Use: To study the reduction of sulphates 

 by Spirillum desulfuricans . Author re- 

 ported that at the end of 5 days there was 

 35 mg. H.iS formed per liter. 



Reference: Beijerinck (1895 p. 54). 



1214. Peklo's Infusion Solution 



Constituents : 



1. Water to 1480.0 cc. 



2. Malt 



3. K2HPO4 8.0 g. 



4. K2CO3 6.0 g. 



