CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



353 



Preparation : 



(1) Add and thoroly mix malt with dis- 

 tilled water. 



(2) Boil for 1 hour and allow to stand at 

 60°C. for 24 hours. 



(3) Boil again for one hour. 



(4) Filter. 



(5) Dilute the filtrate by the addition of a 

 little distilled water. 



(6) After several sterilizations (times or 

 method not specified) and filtering ofi 

 the formed precipitate, distribute in 

 sterile Erlenmeyer flasks. 



(7) To 700.0 cc. of (6) add 780.0 g. of dis- 

 tilled water, 8.0 g. K2HPO4 and 6.0 g. 



of K2CO3. 



(8) The reaction is strongly alkaline. 

 Sterilization decreases the alkalinity 

 somewhat but the reaction still re- 

 mains alkaline. 



Sterilization: Sterilize (7); method not 



given. 

 Use: Cultivation of plant actinomyces. 

 Reference: Peklo (1910 p. 470). 



1215. Bokorny's Malt Infusion Solution 



Constituents : 



1. Water 500.0 cc. 



2. Malt infusion 500.0 cc. 



3. Maltose (2.0%) 20.0 g. 



Preparation : 



(1) Preparation of malt infusion not 

 given. 



(2) Mix (1) with equal parts of water and 

 add 2.0% maltose. 



Sterilization: Not specified. 



Use: Cultivation of yeast. 



Variants: The author used the following: 



(a) Undiluted malt infusion without any 

 maltose. 



(b) Dilute malt infusion with 3 times 

 its volume of water and add 1.0% 

 maltose. 



Reference: Bokorny (1920 p. 30). 



1216. Bacto Malt Extract Broth 

 (Dehydrated) 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Malt e.xtract, dehydrated, 



Bacto 15.0 g. 



Preparation: 



(1) Dissolve 15.0 g. Bacto dehydrated 

 malt extract in 1000.0 cc. of distilled 

 water. 



(2) If sterilized for 20 minutes at 15 

 pounds pressure pH = 4.7d=. 

 Sterilization: Sterilize in the usual manner. 

 Use: Cultivation of yeast. 

 Variants: A more concentrated solution 



may be employed if desired. 

 Reference: Digestive Ferments Co. (1925 



p. 14). 



1217. Reddish's Malt Extract Solution 

 Constituents: 



1. Distilled water 900.0 cc. 



2. Malt extract 100.0 g. 



Preparation : 



(1) Dissolve 100.0 g. dry extract of malt 

 in 900.0 cc. distilled water. 



(2) Make to 8° Kaiser (saccharometer) 

 by adding distilled water (approxi- 

 mately 100.0 cc). 



(3) Adjust the reaction to +1.5 (1.5% 

 agar may be added and dissolved). 



(4) Autoclave for 15 minutes at 15 pounds 

 pressure. 



(5) Filter thru folded filter paper. 



(6) Tube. 



Sterilization: Sterilize at 15 pounds for 



10 minutes or 10 pounds for 15 minutes. 

 Use: Substitute for beer wort. 

 Reference: Reddish (1919 p. 6), 



1218. Park, Williams and Krumwiede's 

 Basal Beer Wort Solution 



Constituents : 



1. Beerwort. 

 Preparation : 



(1) Obtain hopped beerwort from the 

 brewery. 



(2) Autoclave. 



(3) Cool. 



(4) Filter. 



(5) Dissolve 2.0% of one of the added 

 nutrients in the filtrate. 



(6) Tube. 



Sterilization: Sterilization not specified. 

 Use: Cultivation of yeast and molds. 

 Added nutrients: The authors added 2.0% 



of any desired carbohydrate, alcohol, etc. 

 Reference: Park, Williams and Krumwiede 



(1924 p. 134). 



1219. Peklo's Beer Wort Solution 



Constituents : 



1. Distilled water 450.0 cc. 



2. Beer wort 550.0 cc. 



