CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



355 



Use: To determine effect of B. saccharalis 



on composition of cane sugar. 

 Variants : 



(a) The author added 1.0% CaCOj to 

 each flask. 



(b) The author sterilized raw sugar cane 

 juice of a pH about 5.0 on each of 3 

 successive days at 30°C. This me- 

 dium was used to study the produc- 

 tion of gum levan. 



Reference: Owen (1916 p. 242), (1923 

 p. 434). 



1224. Owen's Sulphured Cane Juice 

 Solution 



Constituents: 



1. Sugar cane juice 1000.0 cc. 



2. SO2 (gas) 

 Preparation : 



(1) Pass SO 2 gas into raw sugar cane 

 juice until 10.0 cc. of the juice require 

 5.9 cc. of 0.1 normal NaOH to cause 

 neutralization. 



(2) pH range from 3.8 to 6.9. 

 Sterilization: Sterilize on 3 successive days. 

 Use : To study production of levan by levan 



forming bacteria isolated from raw 

 sugar. Bacillus levaniformans . 



Variants : The author added milk of lime to 

 the above medium until the acidity was 

 reduced from 5.9 cc. — 0.1 normal NaOH 

 to 0.5 cc. NaOH. 



Reference: Owen (1923 p. 434). 



1225. Sherman's Corn Juice 



Constituents: 



1. Corn juice. 

 Preparation : 



(1) Obtain green corn plants in about 

 tosseling stage. 



(2) Pressure applied to get juice. 



(3) Heat in autoclave a few minutes. 



(4) Filter thru filter paper. 



(5) Tube. 



Sterilization: Method not given. 



Use: To show acid production by silage 



organisms. 

 Reference: Sherman (1916 p. 449). 



1226. Buchanan's Silage Infusion Solution 



Constituents : 



1. Water, tap 1000.0 cc. 



2. Silage 500.0 g. 



Preparation : 



(1) Boil 500.0 g. of fresh silage for 

 30 minutes with 1 liter of tap water. 



(2) Filter. Wash the silage on the filter 

 with hot water until a liter of the 

 decoction is secured. 



(3) Filter. 



(4) Distribute in 50.0 cc. lots. 

 Sterilization: Not specified. 



Use: Cultivation of Monascus purpurens 



from silage. 

 Reference: Buchanan (1910 p. 101). 



1227. Reith's Plant Infusion Solution 

 Constituents: 



1. Water, tap 300.0 cc. 



2. Wheat 100.0 g. 



Preparation : 



(1) Soak 100.0 g. wheat in 300.0 g. of tap 

 water for 18 hours. 



(2) Autoclave 30 minutes at 15 pounds. 



(3) Strain thru cheese cloth. 



(4) Adjust to pH = 7.5. 



(5) Tube. 



Sterilization: Sterilize in the autoclave. 



Use: General culture medium. Author 

 reported that the media did not support 

 the growth of Pfeiffer's bacilli unless red 

 blood cells were added, see med. 1228. 



Variants : The author treated rye, beans, 

 peas, or hemp in exactly the same man- 

 ner as wheat above. 



Reference: Reith (1923 p. 245). 



1228. Reith's Blood Cell Plant Infusion 



Enrich medium 1227 by the addition of 

 2.0% red cells of horse blood. 

 Use: Cultivation of Pfeiffer bacillus. 



Gives good growth, but not indol reaction. 

 Reference: Reith (1923 p. 245). 



SUBGROUP I-C. SECTION 16 



Liquid media or basal solutions not con- 

 taining digests, but containing ingredients 

 of animal origin of unknown chemical 

 composition. 

 Ai.* Only one constituent of unknown 



chemical composition present. 

 Bi. Materials of animal origin, exclusive of 



infusions and extracts employed. 

 Ci. Containing animal tissues, cells or 



their derivatives. 



See page 358 for A2 and page 357 for B2 



