358 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Di. Containing no additional organic 



material. 



Biisgen and Hoflich's Meat Extract 

 Solution (Linde) 1349 



Stutzer's Nitrate Meat Extract 



Solution 1350 



D2. Additional organic nitrogen supplied. 



Zike's Basal Glucose Meat Extract 

 Solution 1351 



Stutzer's Glucose Meat Extract 

 Solution 1352 



Hurler's Succinate Meat Extract 

 Solution 1353 



Bloch's Basal Asparagin Meat Ex- 

 tract Solution 1354 



Kappen's Urea Meat Extract Solu- 

 tion 1355 



Viehoever's Urea Meat Extract 

 Solution 1356 



Kappen's Cyanamide Meat Extract 



Solution 1357 



C3. Materials other than infusions or 



extracts employed. 



Homer's Tryptophane Gelatin 

 Solution 1358 



Remy and Rosing's Gelatin Solution. 1359 



Standfuss and Kallert's Bone Jelly 

 Solution 1360 



Berman and Rettger's Gelatin 



Solution 1361 



A2. More than one constituent of unknown 



chemical composition present. 

 Bi. Containing animal cells or tissues. 

 Ci. Animal fluids added. 

 Di. Blood used. 



Fleming's Blood and Minced Meat 

 Medium 1362 



Dean and Nouat's Blood Egg Me- 

 dium (Cutler) 1363 



Bruschettinis Egg Yolk Blood In- 

 fusion Solution 1364 



D2. Serum used. 



Noguchi's Serum Tissue Medium. . . 1365 



Hata's Serum Tissue Medium 1366 



D3. Ascitic or other serous fluids used. 



Noguchi's Ascitic Fluid Tissue 

 Medium 1367 



Kligler and Robertson's Ascitic 



Fluid Egg Medium (Stitt) 1368 



C2. Animal fluids not added. 



Bacto Egg-Meat Medium (De- 

 hydrated) 1369 



Rettger's Egg-Meat Medium 1370 



Besredka's Egg Meat Infusion 

 Medium (BezanQon) 1371 



B2. Not containing animal tissues or 

 cells: containing animal fluids. 

 Ficker's Sputum Serum Solution.... 1372 



Kayser's Blood Bile Solution 1373 



Noguchi's Blood Serum Solution . . . 1374 

 Berman and Rettger's Casein Meat 



Extract Medium 1375 



Adams' Coke Milk Medium 1376 



1229. Harrass' Glucose Liver Medium 



Constituents: 



1. Water 1000.0 cc. 



2. Liver (veal) 1.0 lb. 



3. Glucose (1.0%) 10.0 g. 



Preparation : 



(1) Grind one pound of calf liver in a 

 meat grinding machine. 



(2) Add (1) to 1000.0 cc. of water. 



(3) Add 1.0% glucose (or peptone). 



(4) Mix thoroly. 



(5) Distribute into sterile Erlenmeyer 

 flasks and plug with cotton. 



Sterilization: Sterilize for 1.5 to 2 hours in 



streaming steam. 

 Use: Cultivate obligate anaerobes. 

 Reference: Harrass (1906 p. 2237). 



1230. Harrass' Glucose Brain Medium 



Constituents: 



1. Water 1000.0 cc. 



2. Brain, veal 1.0 lb. 



3. Glucose 10.0 g. 



Preparation : 



(1) Grind one pound of calf brain in a 

 meat grinding machine. 



(2) Add (1) to 1000.0 cc. of water. 



(3) Add 1.0% glucose (or 1.0% peptone) 

 to (2). 



(4) Mix thoroly. 



(5) Distribute into sterile Erlenmeyer 

 flasks and plug with cotton. 



Sterilization: Sterilize for 1.5 to 2.0 hours 



in streaming steam. 

 Use : Cultivation of obligate anaerobes. 

 Reference: Harrass (1906 p. 2237). 



1231. Moon's Brain Medium 



Constituents: 



1. Ringer's solution. 



2. Brain, dog. 

 Preparation : 



(1) Obtain brain material from normal 

 dog under the most careful aseptic 

 precautions. 



